Novel radiosynthesis of PET HSV-tk gene reporter probes [ 18F]FHPG and [18F]FHBG employing dual Sep-Pak SPE techniques

Ji Quan Wang, Qi-Huang Zheng, Xiangshu Fei, Bruce H. Mock, Gary Hutchins

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Positron emission tomography (PET) herpes simplex virus thymidine kinase (HSV-tk) gene reporter probes 9-[(3-[18F]fluoro-1-hydroxy-2-propoxy) methyl]guanine ([18F]FHPG) and 9-(4-[18F]fluoro-3- hydroxymethylbutyl)guanine ([18F]FHBG) were prepared by nucleophilic substitution of the appropriate tosylated precursors with [18F]KF/ Kryptofix 2.2.2 followed by a quick deprotection reaction and purification with a simplified dual Silica Sep-Pak solid-phase extraction (SPE) method in 15-30% radiochemical yield.

Original languageEnglish
Pages (from-to)3933-3938
Number of pages6
JournalBioorganic and Medicinal Chemistry Letters
Volume13
Issue number22
DOIs
StatePublished - Nov 17 2003

Fingerprint

Positron emission tomography
Thymidine Kinase
Solid Phase Extraction
Guanine
Simplexvirus
Reporter Genes
Viruses
Silicon Dioxide
Positron-Emission Tomography
Genes
Purification
Substitution reactions
9-((3-fluoro-1-hydroxy-2-propoxy)methyl)guanine
9-(4-fluoro-3-hydroxymethylbutyl)guanine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

Novel radiosynthesis of PET HSV-tk gene reporter probes [ 18F]FHPG and [18F]FHBG employing dual Sep-Pak SPE techniques. / Wang, Ji Quan; Zheng, Qi-Huang; Fei, Xiangshu; Mock, Bruce H.; Hutchins, Gary.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 13, No. 22, 17.11.2003, p. 3933-3938.

Research output: Contribution to journalArticle

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