Novel Saccharomyces cerevisiae Gene, MRK1, Encoding a Putative Protein Kinase with Similarity to Mammalian Glycogen Synthase Kinase-3 and Drosophila Zeste-White3/Shaggy

T. A. Hardy, D. Wu, Peter Roach

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A Saccharomyces cerevisiae gene was identified that would encode a protein similar to the mammalian protein kinase glycogen synthase kinase-3 (GSK-3) and the Drosophila Zeste-White3/Shaggy gene product. The open reading frame predicts a 375 amino acid polypeptide with a putative protein kinase domain that displays 70% and 39% identity, respectively, to two known yeast proteins, Mds1p and Mck 1p. The new gene, designated MRK1 (Mds1p Related Kinase), is located on chromosome IV. Disruption of MRK1 was not lethal and did not elicit any alteration in glycogen accumulation. In addition, an mck1 mds1 mrk1 triple disruptant was viable.

Original languageEnglish
Pages (from-to)728-734
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume208
Issue number2
DOIs
StatePublished - Mar 17 1995

Fingerprint

Glycogen Synthase Kinase 3
Gene encoding
Yeast
Protein Kinases
Drosophila
Saccharomyces cerevisiae
Genes
Fungal Proteins
Chromosomes
Glycogen
Open Reading Frames
Phosphotransferases
Amino Acids
Peptides
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

@article{550eea733e0042a2b165aaec8ba9e001,
title = "Novel Saccharomyces cerevisiae Gene, MRK1, Encoding a Putative Protein Kinase with Similarity to Mammalian Glycogen Synthase Kinase-3 and Drosophila Zeste-White3/Shaggy",
abstract = "A Saccharomyces cerevisiae gene was identified that would encode a protein similar to the mammalian protein kinase glycogen synthase kinase-3 (GSK-3) and the Drosophila Zeste-White3/Shaggy gene product. The open reading frame predicts a 375 amino acid polypeptide with a putative protein kinase domain that displays 70{\%} and 39{\%} identity, respectively, to two known yeast proteins, Mds1p and Mck 1p. The new gene, designated MRK1 (Mds1p Related Kinase), is located on chromosome IV. Disruption of MRK1 was not lethal and did not elicit any alteration in glycogen accumulation. In addition, an mck1 mds1 mrk1 triple disruptant was viable.",
author = "Hardy, {T. A.} and D. Wu and Peter Roach",
year = "1995",
month = "3",
day = "17",
doi = "10.1006/bbrc.1995.1398",
language = "English",
volume = "208",
pages = "728--734",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Novel Saccharomyces cerevisiae Gene, MRK1, Encoding a Putative Protein Kinase with Similarity to Mammalian Glycogen Synthase Kinase-3 and Drosophila Zeste-White3/Shaggy

AU - Hardy, T. A.

AU - Wu, D.

AU - Roach, Peter

PY - 1995/3/17

Y1 - 1995/3/17

N2 - A Saccharomyces cerevisiae gene was identified that would encode a protein similar to the mammalian protein kinase glycogen synthase kinase-3 (GSK-3) and the Drosophila Zeste-White3/Shaggy gene product. The open reading frame predicts a 375 amino acid polypeptide with a putative protein kinase domain that displays 70% and 39% identity, respectively, to two known yeast proteins, Mds1p and Mck 1p. The new gene, designated MRK1 (Mds1p Related Kinase), is located on chromosome IV. Disruption of MRK1 was not lethal and did not elicit any alteration in glycogen accumulation. In addition, an mck1 mds1 mrk1 triple disruptant was viable.

AB - A Saccharomyces cerevisiae gene was identified that would encode a protein similar to the mammalian protein kinase glycogen synthase kinase-3 (GSK-3) and the Drosophila Zeste-White3/Shaggy gene product. The open reading frame predicts a 375 amino acid polypeptide with a putative protein kinase domain that displays 70% and 39% identity, respectively, to two known yeast proteins, Mds1p and Mck 1p. The new gene, designated MRK1 (Mds1p Related Kinase), is located on chromosome IV. Disruption of MRK1 was not lethal and did not elicit any alteration in glycogen accumulation. In addition, an mck1 mds1 mrk1 triple disruptant was viable.

UR - http://www.scopus.com/inward/record.url?scp=0028899442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028899442&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1995.1398

DO - 10.1006/bbrc.1995.1398

M3 - Article

VL - 208

SP - 728

EP - 734

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -