Novel Saccharomyces cerevisiae Gene, MRK1, encoding a putative protein kinase with similarity to mammalian glycogen synthase kinase-3 and Drosophila zeste-white3/shaggy

Thomas A. Hardy, David Wu, Peter J. Roach

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A Saccharomyces cerevisiae gene was identified that would encode a protein similar to the mammalian protein kinase glycogen synthase kinase-3 (GSK-3) and the Drosophila Zeste-White3/Shaggy gene product. The open reading frame predicts a 375 amino acid polypeptide with a putative protein kinase domain that displays 70% and 39% identity, respectively, to two known yeast proteins, Mds1p and Mck 1p. The new gene, designated MRK1 (Mds1p Related Kinase), is located on chromosome IV. Disruption of MRK1 was not lethal and did not elicit any alteration in glycogen accumulation. In addition, an mck1 mds1 mrk1 triple disruptant was viable.

Original languageEnglish (US)
Pages (from-to)728-734
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume208
Issue number2
DOIs
StatePublished - Mar 17 1995

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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