NtPET: A new application of PET Imaging for characterizing the kinetics of endogenous neurotransmitter release

Evan D. Morris, Karmen Yoder, Chunzhi Wang, Marc D. Normandin, Qi-Huang Zheng, Bruce Mock, Raymond F. Muzic, Janice Froehlich

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We present a new application of positron emission tomography ("ntPET" or "neurotransmitter PET") designed to recover temporal patterns of neurotransmitter release from dynamic data. Our approach employs an enhanced tracer kinetic model that describes uptake of a labeled dopamine D2/D3 receptor ligand in the presence of a time-varying rise and fall in endogenous dopamine. Data must be acquired during both baseline and stimulus (transient dopamine release) conditions. Data from a reference region in both conditions are used as an input function, which alleviates the need for any arterial blood sampling. We use simulation studies to demonstrate the ability of the method to recover the temporal characteristics of an increase in dopamine concentration that might be expected following a drug treatment. The accuracy and precision of the method - as well as its potential for false-positive responses due to noise or changes in blood flow - were examined. Finally, we applied the ntPET method to small-animal imaging data in order to produce the first noninvasive assay of the time-varying release of dopamine in the rat striatum following alcohol.

Original languageEnglish
Pages (from-to)473-489
Number of pages17
JournalMolecular Imaging
Volume4
Issue number4
DOIs
StatePublished - Oct 2005

Fingerprint

Neurotransmitter Agents
Dopamine
Imaging techniques
Kinetics
Dopamine D3 Receptors
Blood
Dopamine D2 Receptors
Drug therapy
Positron emission tomography
Positron-Emission Tomography
Noise
Alcohols
Rats
Ligands
Assays
Animals
Sampling
Pharmaceutical Preparations

Keywords

  • Alcohol
  • Dopamine
  • IndyPET
  • Raclopride
  • Small-animal imaging

ASJC Scopus subject areas

  • Engineering(all)

Cite this

NtPET : A new application of PET Imaging for characterizing the kinetics of endogenous neurotransmitter release. / Morris, Evan D.; Yoder, Karmen; Wang, Chunzhi; Normandin, Marc D.; Zheng, Qi-Huang; Mock, Bruce; Muzic, Raymond F.; Froehlich, Janice.

In: Molecular Imaging, Vol. 4, No. 4, 10.2005, p. 473-489.

Research output: Contribution to journalArticle

Morris, Evan D. ; Yoder, Karmen ; Wang, Chunzhi ; Normandin, Marc D. ; Zheng, Qi-Huang ; Mock, Bruce ; Muzic, Raymond F. ; Froehlich, Janice. / NtPET : A new application of PET Imaging for characterizing the kinetics of endogenous neurotransmitter release. In: Molecular Imaging. 2005 ; Vol. 4, No. 4. pp. 473-489.
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