Nuclear architecture supports integration of physiological regulatory signals for transcription of cell growth and tissue-specific genes during osteoblast differentiation

G. S. Stein, A. J. Van Wijnen, J. L. Stein, J. B. Lian, Joseph Bidwell, M. Montecino

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

During the past several years it has become increasingly evident that the three-dimensional organization of the nucleus plays a critical role in transcriptional control. The principal theme of this prospect will be the contribution of nuclear structure to the regulation of gene expression as functionally related to development and maintenance of the osteoblast phenotype during establishment of bone tissue-like organization. The contributions of nuclear structure as it regulates and is regulated by the progressive developmental expression of cell growth and bone cell related genes will be examined. We will consider signalling mechanisms that integrate the complex and interdependent responsiveness to physiological mediators of osteoblast proliferation and differentiation. The focus will be on the involvement of the nuclear matrix, chromatin structure, and nucleosome organization in transcriptional control of cell growth and bone cell related genes. Findings are presented which are consistent with involvement of nuclear structure in gene regulatory mechanisms which support osteoblast differentiation by addressing four principal questions: 1) Does the representation of nuclear matrix proteins reflect the developmental stage-specific requirements for modifications in transcription during osteoblast differentiation? 2) Are developmental stage-specific transcription factors components of nuclear matrix proteins? 3) Can the nuclear matrix facilitate interrelationships between physiological regulatory signals that control transcription and the integration of activities of multiple promoter regulatory elements? 4) Are alterations in gene expression and cell phenotypic properties in transformed osteoblasts and osteosarcoma cells reflected by modifications in nuclear matrix proteins? There is a striking representation of nuclear matrix proteins unique to cells, tissues as well as developmental stages of differentiation, and tissue organization. Together with selective association of regulatory molecules with the nuclear matrix in a growth and differentiation-specific manner, there is a potential for application of nuclear matrix proteins in tumor diagnosis, assessment of tumor progression, and prognosis of therapies where properties of the transformed state of cells is modified. It is realistic to consider the utilization of nuclear matrix proteins for targeting regions of cell nuclei and specific genomic domains on the basis of developmental phenotypic properties or tissue pathology.

Original languageEnglish (US)
Pages (from-to)4-15
Number of pages12
JournalJournal of Cellular Biochemistry
Volume55
Issue number1
DOIs
StatePublished - 1994

Fingerprint

Nuclear Matrix-Associated Proteins
Osteoblasts
Cell growth
Transcription
Genes
Tissue
Growth
Nuclear Matrix
Bone
Gene expression
Tumors
Bone Development
Nucleosomes
Pathology
Chromatin
Gene Expression Regulation
Protein Transport
Transcription Factors
Osteosarcoma
Regulator Genes

Keywords

  • Cell growth
  • Gene expression
  • Nucleosome
  • Nucleus
  • Osteoblast

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Nuclear architecture supports integration of physiological regulatory signals for transcription of cell growth and tissue-specific genes during osteoblast differentiation. / Stein, G. S.; Van Wijnen, A. J.; Stein, J. L.; Lian, J. B.; Bidwell, Joseph; Montecino, M.

In: Journal of Cellular Biochemistry, Vol. 55, No. 1, 1994, p. 4-15.

Research output: Contribution to journalArticle

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