Nuclear magnetic resonance spectroscopy-based metabolomics of the fatty pancreas: Implicating fat in pancreatic pathology

Nicholas Zyromski, Abhishek Mathur, G. A Nagana Gowda, Carl Murphy, Deborah A. Swartz-Basile, Terence E. Wade, Henry A. Pitt, Daniel Raftery

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: Obesity is a worldwide epidemic and a significant risk factor for pancreatic diseases including pancreatitis and pancreatic cancer; the mechanisms underlying this association are unknown. Metabolomics is a powerful new analytical approach for describing the metabolome (compliment of small molecules) of cells, tissue or biofluids at any given time. Our aim was to analyze pancreatic fat content in lean and congenitally obese mice using both metabolomic analysis and conventional chromatography. Methods: The pancreatic fat content of 12 lean (C57BL/6J), 12 obese leptin-deficient (Lepob) and 12 obese hyperleptinemic (Lepdb) mice was evaluated by metabolomic analysis, thin-layer and gas chromatography. Results: Pancreata of congenitally obese mice had significantly more total pancreatic fat, triglycerides and free fatty acids, but significantly less phospholipids and cholesterol than those of lean mice. Metabolomic analysis showed excellent correlation with thin-layer and gas chromatography in measuring total fat, triglycerides and phospholipids. Conclusions: Differences in pancreatic fat content and character may have important implications when considering the local pancreatic proinflammatory milieu in obesity. Metabolomic analysis is a valid, powerful tool with which to further define the mechanisms by which fat impacts pancreatic disease.

Original languageEnglish
Pages (from-to)410-419
Number of pages10
JournalPancreatology
Volume9
Issue number4
DOIs
StatePublished - Jun 2009

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Metabolomics
Pancreas
Magnetic Resonance Spectroscopy
Fats
Pathology
Obese Mice
Pancreatic Diseases
Thin Layer Chromatography
Gas Chromatography
Phospholipids
Triglycerides
Obesity
Metabolome
Leptin
Pancreatic Neoplasms
Nonesterified Fatty Acids
Pancreatitis
Chromatography
Cholesterol

Keywords

  • Inflammation
  • Metabolomics
  • Obesity
  • Pancreatic cancer
  • Pancreatitis

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Hepatology

Cite this

Zyromski, N., Mathur, A., Gowda, G. A. N., Murphy, C., Swartz-Basile, D. A., Wade, T. E., ... Raftery, D. (2009). Nuclear magnetic resonance spectroscopy-based metabolomics of the fatty pancreas: Implicating fat in pancreatic pathology. Pancreatology, 9(4), 410-419. https://doi.org/10.1159/000199436

Nuclear magnetic resonance spectroscopy-based metabolomics of the fatty pancreas : Implicating fat in pancreatic pathology. / Zyromski, Nicholas; Mathur, Abhishek; Gowda, G. A Nagana; Murphy, Carl; Swartz-Basile, Deborah A.; Wade, Terence E.; Pitt, Henry A.; Raftery, Daniel.

In: Pancreatology, Vol. 9, No. 4, 06.2009, p. 410-419.

Research output: Contribution to journalArticle

Zyromski, N, Mathur, A, Gowda, GAN, Murphy, C, Swartz-Basile, DA, Wade, TE, Pitt, HA & Raftery, D 2009, 'Nuclear magnetic resonance spectroscopy-based metabolomics of the fatty pancreas: Implicating fat in pancreatic pathology', Pancreatology, vol. 9, no. 4, pp. 410-419. https://doi.org/10.1159/000199436
Zyromski, Nicholas ; Mathur, Abhishek ; Gowda, G. A Nagana ; Murphy, Carl ; Swartz-Basile, Deborah A. ; Wade, Terence E. ; Pitt, Henry A. ; Raftery, Daniel. / Nuclear magnetic resonance spectroscopy-based metabolomics of the fatty pancreas : Implicating fat in pancreatic pathology. In: Pancreatology. 2009 ; Vol. 9, No. 4. pp. 410-419.
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