Nuclear Matrix Association of Multiple Sequence-Specific DNA Binding Activities Related to SP-1, ATF, CCAAT, C/EBP, OCT-1, and AP-1

André J. van Wijnen, Joseph Bidwell, Edward G. Fey, Sheldon Penman, Jane B. Lian, Janet L. Stein, Gary S. Stein

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

The association of DNA binding proteins with the nuclear matrix may be related to a functional role of this subcellular structure in chromatin organization and gene regulation. In this study, nuclear matrix preparations from human HeLa S3 cervical carcinoma and rat ROS 17/2.8 osteosarcoma cells were assayed for the presence of DNA binding activities using consensus binding sequences of well-characterized transcription factors as probes. Competition analysis shows that each probe interacts with different nuclear matrix proteins in a sequence-specific manner and that DNA binding activities related to or identical with SP-1, ATF, CCAAT, C/EBP, OCT-1, and AP-1 are present in the nuclear matrix fraction of different cell types. Comparison of the relative abundance of these transcription factor binding activities in nuclear matrix and nonmatrix nuclear fractions suggests that the distribution between these two fractions is cell type specific, cell growth dependent, or independent of these biological parameters. These results are consistent with the postulated role of the nuclear matrix in transcriptional regulation of gene expression.

Original languageEnglish (US)
Pages (from-to)8397-8402
Number of pages6
JournalBiochemistry
Volume32
Issue number33
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Nuclear Matrix
Transcription Factor AP-1
Gene expression
Transcription Factors
Nuclear Matrix-Associated Proteins
DNA
DNA-Binding Proteins
Cell growth
Chromatin
Rats
Consensus Sequence
Gene Expression Regulation
Osteosarcoma
Carcinoma
Growth
Genes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Nuclear Matrix Association of Multiple Sequence-Specific DNA Binding Activities Related to SP-1, ATF, CCAAT, C/EBP, OCT-1, and AP-1. / van Wijnen, André J.; Bidwell, Joseph; Fey, Edward G.; Penman, Sheldon; Lian, Jane B.; Stein, Janet L.; Stein, Gary S.

In: Biochemistry, Vol. 32, No. 33, 01.01.1993, p. 8397-8402.

Research output: Contribution to journalArticle

van Wijnen, André J. ; Bidwell, Joseph ; Fey, Edward G. ; Penman, Sheldon ; Lian, Jane B. ; Stein, Janet L. ; Stein, Gary S. / Nuclear Matrix Association of Multiple Sequence-Specific DNA Binding Activities Related to SP-1, ATF, CCAAT, C/EBP, OCT-1, and AP-1. In: Biochemistry. 1993 ; Vol. 32, No. 33. pp. 8397-8402.
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