Nuclear Matrix Association: Switching to the Invasive Cytotrophoblast

K. J. Drennan, A. K. Linnemann, A. E. Platts, H. H. Heng, D. R. Armant, S. A. Krawetz

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Abnormal trophoblast invasion is associated with the most common and most severe complications of human pregnancy. The biology of invasion, as well as the etiology of abnormal invasion remains poorly understood. The aim of this study was to characterize the transcriptome of the HTR-8/SVneo human cytotrophoblast cell line which displays well characterized invasive and non-invasive behavior, and to correlate the activity of the transcriptome with nuclear matrix attachment and cell phenotype. Comparison of the invasive to non-invasive HTR transcriptomes was unremarkable. In contrast, comparison of the MARs on chromosomes 14-18 revealed an increased number of MARs associated with the invasive phenotype. These attachment areas were more likely to be associated with silent rather than actively transcribed genes. This study supports the view that nuclear matrix attachment may play an important role in cytotrophoblast invasion by ensuring specific silencing that facilitates invasion.

Original languageEnglish (US)
Pages (from-to)365-372
Number of pages8
JournalPlacenta
Volume31
Issue number5
DOIs
StatePublished - May 1 2010

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Keywords

  • Epigenetics
  • Gene expression
  • Invasion
  • Nuclear matrix
  • Trophoblast

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

Cite this

Drennan, K. J., Linnemann, A. K., Platts, A. E., Heng, H. H., Armant, D. R., & Krawetz, S. A. (2010). Nuclear Matrix Association: Switching to the Invasive Cytotrophoblast. Placenta, 31(5), 365-372. https://doi.org/10.1016/j.placenta.2010.02.012