Nuclear matrix-intermediate filament proteins of the dental follicle/enamel epithelium and their changes during tooth eruption in dogs

Joseph Bidwell, Edward G. Fey, Sandy C. Marks

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Tooth eruption activates a localized resorption and formation of alveolar bone and these activities depend upon the adjacent parts, coronal and basal, respectively, of the dental follicle-enamel epithelium. In this study the nuclear matrix-intermediate filament (NM-IF) proteins of these tissues were isolated in order to continue investigations into the molecular mechanisms underlying eruption. Dental follicles were removed from the third and fourth premolar of dogs at 13, 16 and 20 weeks (pre-, early, and mid-to-late eruption of these teeth) and NM-IF proteins were extracted from the coronal and basal halves. Most of the NM-IF protein profiles of these coronal and basal parts on one-dimensional, sodium dodecyl sulphate-polyacrylamide gel electrophoresis were remarkably constant, indicating an essentially uniform cellular composition. However, differences between these tissues were observed and some of these changed during eruption. Based on recent observations that nuclear matrix changes reflect and may even mediate cell-specific changes in gene expression, these findings suggest that changes in nuclear matrix proteins may be related to the molecular basis for some aspects of differential gene expression in the coronal and basal regions of the dental follicle and account for the ability of these tissues to activate bone resorption and formation during tooth eruption.

Original languageEnglish
Pages (from-to)1047-1051
Number of pages5
JournalArchives of Oral Biology
Volume40
Issue number11
DOIs
StatePublished - 1995

Fingerprint

Dental Sac
Tooth Eruption
Intermediate Filament Proteins
Nuclear Matrix
Dental Enamel
Epithelium
Dogs
Osteogenesis
Nuclear Matrix-Associated Proteins
Alveolar Bone Loss
Gene Expression
Bicuspid
Bone Resorption
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis

Keywords

  • dental follicle
  • nuclear matrix proteins
  • tissue matrix
  • tooth eruption

ASJC Scopus subject areas

  • Cell Biology
  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Nuclear matrix-intermediate filament proteins of the dental follicle/enamel epithelium and their changes during tooth eruption in dogs. / Bidwell, Joseph; Fey, Edward G.; Marks, Sandy C.

In: Archives of Oral Biology, Vol. 40, No. 11, 1995, p. 1047-1051.

Research output: Contribution to journalArticle

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