Nuclear matrix proteins distinguish normal diploid osteoblasts from osteosarcoma cells

Joseph Bidwell, Edward G. Fey, André J. Van Wijnen, Sheldon Penman, Janet L. Stein, Jane B. Lian, Gary S. Stein

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Interrelationships between nuclear architecture and gene expression were examined by comparing the representation of nuclear matrix proteins in ROS 17/2.8 rat and MG-63 human osteosarcoma cells with those in normal diploid osteoblasts. The tumor-derived cells coexpress genes which are expressed in a sequential and mutually exclusive manner during the progressive stages of osteoblast differentiation. In osteosarcoma cells two-dimensional electrophoretic analysis indicates a composite representation of nuclear matrix proteins characteristic of both the proliferative and postproliferative periods of osteoblast phenotype development. In addition, nuclear matrix proteins unique to the tumor cells and the absence of nuclear matrix proteins found only in normal diploid osteoblasts are observed. Tumor- specific nuclear matrix proteins include those expressed in a proliferation- dependent and independent manner. There is a parallel relationship between nuclear matrix proteins and the expression of cell growth and tissue-specific genes during osteoblast differentiation and in osteosarcoma cells where the developmental sequence of gene expression has been abrogated. Nuclear matrix proteins therefore provide markers reflecting defined periods of bone cell differentiation and phenotypic characteristics of an osteosarcoma.

Original languageEnglish
Pages (from-to)28-32
Number of pages5
JournalCancer Research
Volume54
Issue number1
StatePublished - Jan 1 1994

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Nuclear Matrix-Associated Proteins
Osteosarcoma
Diploidy
Osteoblasts
Developmental Genes
Gene Expression
Neoplasms
Genes
Cell Differentiation
Phenotype
Bone and Bones
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Bidwell, J., Fey, E. G., Van Wijnen, A. J., Penman, S., Stein, J. L., Lian, J. B., & Stein, G. S. (1994). Nuclear matrix proteins distinguish normal diploid osteoblasts from osteosarcoma cells. Cancer Research, 54(1), 28-32.

Nuclear matrix proteins distinguish normal diploid osteoblasts from osteosarcoma cells. / Bidwell, Joseph; Fey, Edward G.; Van Wijnen, André J.; Penman, Sheldon; Stein, Janet L.; Lian, Jane B.; Stein, Gary S.

In: Cancer Research, Vol. 54, No. 1, 01.01.1994, p. 28-32.

Research output: Contribution to journalArticle

Bidwell, J, Fey, EG, Van Wijnen, AJ, Penman, S, Stein, JL, Lian, JB & Stein, GS 1994, 'Nuclear matrix proteins distinguish normal diploid osteoblasts from osteosarcoma cells', Cancer Research, vol. 54, no. 1, pp. 28-32.
Bidwell J, Fey EG, Van Wijnen AJ, Penman S, Stein JL, Lian JB et al. Nuclear matrix proteins distinguish normal diploid osteoblasts from osteosarcoma cells. Cancer Research. 1994 Jan 1;54(1):28-32.
Bidwell, Joseph ; Fey, Edward G. ; Van Wijnen, André J. ; Penman, Sheldon ; Stein, Janet L. ; Lian, Jane B. ; Stein, Gary S. / Nuclear matrix proteins distinguish normal diploid osteoblasts from osteosarcoma cells. In: Cancer Research. 1994 ; Vol. 54, No. 1. pp. 28-32.
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