Nucleotide depletion increases trafficking of gentamicin to the Golgi complex in LLC-PK1 cells

Ruben M. Sandoval, Robert Bacallao, Kenneth Dunn, Jeffrey D. Leiser, Bruce Molitoris

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Having shown rapid trafficking of aminoglycosides to the Golgi complex in cell culture, we focused on the injurious interaction that occurs when gentamicin administration is preceded by renal ischemia. Using Texas red-labeled gentamicin as a tracer, we determined that 15 min of cellular nucleotide depletion did not significantly increase subsequent uptake. However, cells previously depleted of nucleotides accumulated significantly more Texas red-labeled gentamicin within a dispersed Golgi complex. Using Ricinus communis and Lens culinaris lectins, which label specific compartments of the Golgi complex (trans-Golgi network/trans and medial/cis compartments, respectively), we determined that the medial/cis compartment dispersed after 15 min of nucleotide depletion but the trans-Golgi network/trans compartment remained unaffected. An increase in the number of cells exhibiting disrupted medial/cis-Golgi morphology after repletion in physiological media containing gentamicin was also seen. In summary, the increase in nephrotoxicity seen when ischemia precedes aminoglycoside uptake may be part of a complex mechanism initially involving increased Golgi accumulation and prolonged Golgi dispersion. The Golgi complex must then endure the effects of gentamicin accumulated in larger quantities in an aberrant physiological state.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Physiology
Volume283
Issue number6 52-6
StatePublished - Dec 1 2002

Fingerprint

LLC-PK1 Cells
Golgi Apparatus
Gentamicins
Nucleotides
trans-Golgi Network
Aminoglycosides
Ischemia
Ricinus
Cell Culture Techniques
Cell Count
Kidney

Keywords

  • Acute renal failure
  • Aminoglycosides
  • ATP depletion
  • Cell toxicity
  • Ischemia
  • Nephrotoxicity
  • Proximal tubule cells

ASJC Scopus subject areas

  • Physiology

Cite this

Nucleotide depletion increases trafficking of gentamicin to the Golgi complex in LLC-PK1 cells. / Sandoval, Ruben M.; Bacallao, Robert; Dunn, Kenneth; Leiser, Jeffrey D.; Molitoris, Bruce.

In: American Journal of Physiology - Renal Physiology, Vol. 283, No. 6 52-6, 01.12.2002.

Research output: Contribution to journalArticle

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