Nucleotide receptor P2u partially mediates ATP-induced cell cycle progression of aortic smooth muscle cells

Rabé Malam-Souley, Cheikh Seye, Alain Pierre Gadeau, Gervaise Loirand, Xavier Pillois, Michel Campan, Pierre Pacaud, Claude Desgranges

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

mRNA of the P2u purinoceptor (or nucleotide receptor) is detected both by polymerase chain reaction or Northern blot analyses in cultured aortic smooth muscle cells. When added to the culture medium of these cells, UTP, a specific ligand of the P2u receptor, induces an increased expression of both immediate-early and delayed-early cell cycle-dependent genes. This induction demonstrates similar features (kinetics, concentration dependence) to those obtained after stimulation of aortic smooth cells by exogenous ATP, a common ligand for most P2 purinoceptors. In contrast, 2-methylthioATP, a preferential ligand for P2y purinoceptors, induces only a significant increase of immediate-early genes but not of delayed-early genes. Moreover, the 2-methylthioATP-induced responses (c-fos mRNA increase, free intracellular calcium transient) are lower than those induced by ATP or UTP and are complementary to those of UTP. These results demonstrate that functional P2u receptors are present on cultured aortic smooth muscle cells and suggest that the bulk of responses induced by extracellular ATP on cell cycle progression are mediated via P2u purinoceptors, a hypothesis confirmed by cytofluorometric studies. Since some ATP- or UTP-induced genes code for chemotactic proteins (monocyte chemoattractant protein-1 and osteopontin), this study suggests that these nucleotides may contribute to vascular or blood cell migration and proliferation and consequently to the genesis of arterial diseases.

Original languageEnglish (US)
Pages (from-to)57-65
Number of pages9
JournalJournal of Cellular Physiology
Volume166
Issue number1
StatePublished - Jan 1996
Externally publishedYes

Fingerprint

Uridine Triphosphate
Purinergic Receptors
Smooth Muscle Myocytes
Muscle
Cell Cycle
Nucleotides
Adenosine Triphosphate
Cells
Genes
Ligands
Purinergic P2 Receptors
cdc Genes
Messenger RNA
Osteopontin
Immediate-Early Genes
Chemokine CCL2
Northern Blotting
Polymerase chain reaction
Cell Movement
Blood Vessels

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Malam-Souley, R., Seye, C., Gadeau, A. P., Loirand, G., Pillois, X., Campan, M., ... Desgranges, C. (1996). Nucleotide receptor P2u partially mediates ATP-induced cell cycle progression of aortic smooth muscle cells. Journal of Cellular Physiology, 166(1), 57-65.

Nucleotide receptor P2u partially mediates ATP-induced cell cycle progression of aortic smooth muscle cells. / Malam-Souley, Rabé; Seye, Cheikh; Gadeau, Alain Pierre; Loirand, Gervaise; Pillois, Xavier; Campan, Michel; Pacaud, Pierre; Desgranges, Claude.

In: Journal of Cellular Physiology, Vol. 166, No. 1, 01.1996, p. 57-65.

Research output: Contribution to journalArticle

Malam-Souley, R, Seye, C, Gadeau, AP, Loirand, G, Pillois, X, Campan, M, Pacaud, P & Desgranges, C 1996, 'Nucleotide receptor P2u partially mediates ATP-induced cell cycle progression of aortic smooth muscle cells', Journal of Cellular Physiology, vol. 166, no. 1, pp. 57-65.
Malam-Souley, Rabé ; Seye, Cheikh ; Gadeau, Alain Pierre ; Loirand, Gervaise ; Pillois, Xavier ; Campan, Michel ; Pacaud, Pierre ; Desgranges, Claude. / Nucleotide receptor P2u partially mediates ATP-induced cell cycle progression of aortic smooth muscle cells. In: Journal of Cellular Physiology. 1996 ; Vol. 166, No. 1. pp. 57-65.
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