Nucleotide sequence of the inverted terminal repetition in adeno-associated virus DNA

E. Lusby, K. H. Fife, K. I. Berns

Research output: Contribution to journalArticle

113 Scopus citations

Abstract

The inverted terminal repetition in adeno-associated virus type 2 DNA has been sequenced. The terminal repetition contains 145 nucleotides of which the first 125 nucleotides can self-base pair to form a T-shaped hairpin structure. Both restriction endonuclease analysis with SmaI and BglI and direct sequence analysis of the SmaI fragments provide evidence for two sequences in the region of the terminal repetition between nucleotides 44 and 81. The two sequences represent an inversion of the first 125 nucleotides of the terminal repetition. Based on these data a model for adeno-associated virus DNA replication is presented which agrees in detail with a general model for eucaryotic DNA replication originally proposed by Cavalier-Smith (T. Cavalier-Smith, Nature [London] 18:672-684, 1976).

Original languageEnglish (US)
Pages (from-to)402-409
Number of pages8
JournalJournal of virology
Volume34
Issue number2
DOIs
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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