Nucleus-specific importin alpha proteins and nucleoporins regulate protein import and nuclear division in the binucleate Tetrahymena thermophila

Colin D. Malone, Katarzyna A. Falkowska, Alanna Y. Li, Sarah E. Galanti, Reshi C. Kanuru, Elizabeth G. LaMont, Kate C. Mazzarella, Alan J. Micev, Morwan M. Osman, Nicholas K. Piotrowski, Jason W. Suszko, Adam C. Timm, Ming Ming Xu, Lucy Liu, Douglas L. Chalker

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The ciliate Tetrahymena thermophila, having both germ line micronuclei and somatic macronuclei, must possess a specialized nucleocytoplasmic transport system to import proteins into the correct nucleus. To understand how Tetrahymena can target proteins to distinct nuclei, we first characterized FG repeat-containing nucleoporins and found that micro- and macronuclei utilize unique subsets of these proteins. This finding implicates these proteins in the differential permeability of the two nuclei and implies that nuclear pores with discrete specificities are assembled within a single cell. To identify the import machineries that interact with these different pores, we characterized the large families of karyopherin homologs encoded within the genome. Localization studies of 13 putative importin (imp) α- and 11 imp β-like proteins revealed that imp β-like proteins are nucleus specific-nine localized to the germ line micronucleus-but that most imp β-like proteins localized to both types of nuclei. These data suggest that micronucleus-specific proteins are transported by specific imp α adapters. The different imp α proteins exhibit substantial sequence divergence and do not appear to be simply redundant in function. Disruption of the IMA10 gene encoding an imp α-like protein that accumulates in dividing micronuclei results in nuclear division defects and lethality. Thus, nucleus-specific protein import and nuclear function in Tetrahymena are regulated by diverse, specialized karyopherins.

Original languageEnglish (US)
Pages (from-to)1487-1499
Number of pages13
JournalEukaryotic Cell
Volume7
Issue number9
DOIs
StatePublished - Sep 2008
Externally publishedYes

Fingerprint

alpha Karyopherins
importins
Nuclear Pore Complex Proteins
Tetrahymena thermophila
nucleoporins
Cell Nucleus Division
Karyopherins
imports
Proteins
proteins
karyopherins
Macronucleus
Tetrahymena
Cell Nucleus Active Transport
Germ Cells
germ cells
nucleocytoplasmic transport
Nuclear Pore
Nuclear Proteins
nuclear membrane

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Malone, C. D., Falkowska, K. A., Li, A. Y., Galanti, S. E., Kanuru, R. C., LaMont, E. G., ... Chalker, D. L. (2008). Nucleus-specific importin alpha proteins and nucleoporins regulate protein import and nuclear division in the binucleate Tetrahymena thermophila. Eukaryotic Cell, 7(9), 1487-1499. https://doi.org/10.1128/EC.00193-08

Nucleus-specific importin alpha proteins and nucleoporins regulate protein import and nuclear division in the binucleate Tetrahymena thermophila. / Malone, Colin D.; Falkowska, Katarzyna A.; Li, Alanna Y.; Galanti, Sarah E.; Kanuru, Reshi C.; LaMont, Elizabeth G.; Mazzarella, Kate C.; Micev, Alan J.; Osman, Morwan M.; Piotrowski, Nicholas K.; Suszko, Jason W.; Timm, Adam C.; Xu, Ming Ming; Liu, Lucy; Chalker, Douglas L.

In: Eukaryotic Cell, Vol. 7, No. 9, 09.2008, p. 1487-1499.

Research output: Contribution to journalArticle

Malone, CD, Falkowska, KA, Li, AY, Galanti, SE, Kanuru, RC, LaMont, EG, Mazzarella, KC, Micev, AJ, Osman, MM, Piotrowski, NK, Suszko, JW, Timm, AC, Xu, MM, Liu, L & Chalker, DL 2008, 'Nucleus-specific importin alpha proteins and nucleoporins regulate protein import and nuclear division in the binucleate Tetrahymena thermophila', Eukaryotic Cell, vol. 7, no. 9, pp. 1487-1499. https://doi.org/10.1128/EC.00193-08
Malone, Colin D. ; Falkowska, Katarzyna A. ; Li, Alanna Y. ; Galanti, Sarah E. ; Kanuru, Reshi C. ; LaMont, Elizabeth G. ; Mazzarella, Kate C. ; Micev, Alan J. ; Osman, Morwan M. ; Piotrowski, Nicholas K. ; Suszko, Jason W. ; Timm, Adam C. ; Xu, Ming Ming ; Liu, Lucy ; Chalker, Douglas L. / Nucleus-specific importin alpha proteins and nucleoporins regulate protein import and nuclear division in the binucleate Tetrahymena thermophila. In: Eukaryotic Cell. 2008 ; Vol. 7, No. 9. pp. 1487-1499.
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