Nutritional regulation of the protein kinases responsible for the phosphorylation of the α-ketoacid dehydrogenase complexes

R. A. Harris, K. M. Popov, Y. Zhao

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The branched-chain α-ketoacid dehydrogenase (BCKDH) and pyruvate dehydrogenase (PDH) complexes are regulated by phosphorylation cycles catalyzed by complex-specific protein kinases and phosphoprotein phosphatases. Molecular cloning of these mitochondrial protein kinases has established a new family of protein kinases in eukaryotes that appears related by primary sequence to the histidine protein kinase family of prokaryotes. Changes in the activities of both kinases that are stable, i.e., not caused directly by allosteric effectors, correlate inversely with the changes in the activity states of the complexes that occur in different nutritional states. For example, BCKDH kinase activity is increased and the BCKDH complex activity state is decreased in rats fed diets deficient in protein. The increase in BCKDH kinase activity is due to an increase in the amount of BCKDH kinase protein bound to the BCKDH complex. The message level for BCKDH kinase also increases in the liver of rats starved for protein, suggesting a pretranslational mechanism exists for the long-term regulation of BCKDH kinase. Starvation and high-fat feeding cause a stable increase in PDH kinase activity and a corresponding decrease in activity state of the PDH complex. The mechanism responsible has not been defined.

Original languageEnglish (US)
Pages (from-to)1758S-1761S
JournalJournal of Nutrition
Volume125
Issue number6 SUPPL.
StatePublished - Jan 1 1995

Fingerprint

3-methyl-2-oxobutanoate dehydrogenase (lipoamide)
3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
protein kinases
Protein Kinases
phosphorylation
Oxidoreductases
Phosphorylation
phosphotransferases (kinases)
Phosphotransferases
pyruvate dehydrogenase (lipoamide)
Pyruvate Dehydrogenase Complex
Phosphoprotein Phosphatases
protein-histidine kinases
pyruvate dehydrogenase (acetyl-transferring) kinase
phosphoprotein phosphatase
new family
Mitochondrial Proteins
rats
Molecular Cloning
prokaryotic cells

Keywords

  • branched chain amino acids
  • kinases
  • leucine
  • pyruvate
  • pyruvate dehydrogenase

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Nutritional regulation of the protein kinases responsible for the phosphorylation of the α-ketoacid dehydrogenase complexes. / Harris, R. A.; Popov, K. M.; Zhao, Y.

In: Journal of Nutrition, Vol. 125, No. 6 SUPPL., 01.01.1995, p. 1758S-1761S.

Research output: Contribution to journalArticle

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