Obesity is associated with increased levels of circulating hepatocyte growth factor

Jalees Rehman, Robert V. Considine, Jason E. Bovenkerk, Jingling Li, Catharine A. Slavens, Rose Marie Jones, Keith L. March

Research output: Contribution to journalReview article

116 Citations (Scopus)

Abstract

OBJECTIVES: This study evaluated whether obesity in humans was associated with an increase in circulating hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) levels. BACKGROUND: Obesity acts as a cardiovascular risk factor by mechanisms that are not fully understood. Adipose tissue is able to secrete multiple cytokines and growth factors ex vivo. We hypothesized that the increased presence of adipose tissue in obese subjects results in systemic elevations of the mitogenic factors HGF and VEGF. METHODS: Blood samples were obtained from lean (n = 21) and obese (n = 44) volunteers. Serum HGF and VEGF levels were assessed by enzyme-linked immunoadsorbent assay. Insulin and fasting glucose levels were measured to evaluate insulin sensitivity. Conditioned medium of adipose cells was assayed for HGF secretion. RESULTS: Serum HGF levels in obese subjects were more than three-fold higher than those of lean subjects (2,462 ± 184 pg/ml vs. 765 ± 48 pg/ml, p < 0.0001). The VEGF levels were not significantly elevated in obese subjects (135 ± 31 pg/ml vs. 128 ± 37 pg/ml). The HGF concentrations, but not VEGF concentrations, were significantly correlated with body mass index (BMI) (p < 0.0001, r = 0.74). The observed increases in HGF concentrations of obese subjects were not secondary to insulin resistance or hypertension. Freshly isolated human adipose cells secreted HGF. CONCLUSIONS: Our results indicate that obesity is associated with a marked increase in circulating HGF levels, which correlate linearly with BMI. Because vascular growth factors have been associated with the pathogenesis of atherosclerosis, the possible role of such humoral factors as a link between obesity and cardiovascular disease is very intriguing.

Original languageEnglish (US)
Pages (from-to)1408-1413
Number of pages6
JournalJournal of the American College of Cardiology
Volume41
Issue number8
DOIs
StatePublished - Apr 2003

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Hepatocyte Growth Factor
Obesity
Vascular Endothelial Growth Factor A
Insulin Resistance
Adipose Tissue
Intercellular Signaling Peptides and Proteins
Body Mass Index
Immunosorbents
Conditioned Culture Medium
Serum
Volunteers
Fasting
Atherosclerosis
Cardiovascular Diseases
Insulin
Cytokines
Hypertension
Glucose

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Obesity is associated with increased levels of circulating hepatocyte growth factor. / Rehman, Jalees; Considine, Robert V.; Bovenkerk, Jason E.; Li, Jingling; Slavens, Catharine A.; Jones, Rose Marie; March, Keith L.

In: Journal of the American College of Cardiology, Vol. 41, No. 8, 04.2003, p. 1408-1413.

Research output: Contribution to journalReview article

Rehman, Jalees ; Considine, Robert V. ; Bovenkerk, Jason E. ; Li, Jingling ; Slavens, Catharine A. ; Jones, Rose Marie ; March, Keith L. / Obesity is associated with increased levels of circulating hepatocyte growth factor. In: Journal of the American College of Cardiology. 2003 ; Vol. 41, No. 8. pp. 1408-1413.
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abstract = "OBJECTIVES: This study evaluated whether obesity in humans was associated with an increase in circulating hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) levels. BACKGROUND: Obesity acts as a cardiovascular risk factor by mechanisms that are not fully understood. Adipose tissue is able to secrete multiple cytokines and growth factors ex vivo. We hypothesized that the increased presence of adipose tissue in obese subjects results in systemic elevations of the mitogenic factors HGF and VEGF. METHODS: Blood samples were obtained from lean (n = 21) and obese (n = 44) volunteers. Serum HGF and VEGF levels were assessed by enzyme-linked immunoadsorbent assay. Insulin and fasting glucose levels were measured to evaluate insulin sensitivity. Conditioned medium of adipose cells was assayed for HGF secretion. RESULTS: Serum HGF levels in obese subjects were more than three-fold higher than those of lean subjects (2,462 ± 184 pg/ml vs. 765 ± 48 pg/ml, p < 0.0001). The VEGF levels were not significantly elevated in obese subjects (135 ± 31 pg/ml vs. 128 ± 37 pg/ml). The HGF concentrations, but not VEGF concentrations, were significantly correlated with body mass index (BMI) (p < 0.0001, r = 0.74). The observed increases in HGF concentrations of obese subjects were not secondary to insulin resistance or hypertension. Freshly isolated human adipose cells secreted HGF. CONCLUSIONS: Our results indicate that obesity is associated with a marked increase in circulating HGF levels, which correlate linearly with BMI. Because vascular growth factors have been associated with the pathogenesis of atherosclerosis, the possible role of such humoral factors as a link between obesity and cardiovascular disease is very intriguing.",
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T1 - Obesity is associated with increased levels of circulating hepatocyte growth factor

AU - Rehman, Jalees

AU - Considine, Robert V.

AU - Bovenkerk, Jason E.

AU - Li, Jingling

AU - Slavens, Catharine A.

AU - Jones, Rose Marie

AU - March, Keith L.

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N2 - OBJECTIVES: This study evaluated whether obesity in humans was associated with an increase in circulating hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) levels. BACKGROUND: Obesity acts as a cardiovascular risk factor by mechanisms that are not fully understood. Adipose tissue is able to secrete multiple cytokines and growth factors ex vivo. We hypothesized that the increased presence of adipose tissue in obese subjects results in systemic elevations of the mitogenic factors HGF and VEGF. METHODS: Blood samples were obtained from lean (n = 21) and obese (n = 44) volunteers. Serum HGF and VEGF levels were assessed by enzyme-linked immunoadsorbent assay. Insulin and fasting glucose levels were measured to evaluate insulin sensitivity. Conditioned medium of adipose cells was assayed for HGF secretion. RESULTS: Serum HGF levels in obese subjects were more than three-fold higher than those of lean subjects (2,462 ± 184 pg/ml vs. 765 ± 48 pg/ml, p < 0.0001). The VEGF levels were not significantly elevated in obese subjects (135 ± 31 pg/ml vs. 128 ± 37 pg/ml). The HGF concentrations, but not VEGF concentrations, were significantly correlated with body mass index (BMI) (p < 0.0001, r = 0.74). The observed increases in HGF concentrations of obese subjects were not secondary to insulin resistance or hypertension. Freshly isolated human adipose cells secreted HGF. CONCLUSIONS: Our results indicate that obesity is associated with a marked increase in circulating HGF levels, which correlate linearly with BMI. Because vascular growth factors have been associated with the pathogenesis of atherosclerosis, the possible role of such humoral factors as a link between obesity and cardiovascular disease is very intriguing.

AB - OBJECTIVES: This study evaluated whether obesity in humans was associated with an increase in circulating hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) levels. BACKGROUND: Obesity acts as a cardiovascular risk factor by mechanisms that are not fully understood. Adipose tissue is able to secrete multiple cytokines and growth factors ex vivo. We hypothesized that the increased presence of adipose tissue in obese subjects results in systemic elevations of the mitogenic factors HGF and VEGF. METHODS: Blood samples were obtained from lean (n = 21) and obese (n = 44) volunteers. Serum HGF and VEGF levels were assessed by enzyme-linked immunoadsorbent assay. Insulin and fasting glucose levels were measured to evaluate insulin sensitivity. Conditioned medium of adipose cells was assayed for HGF secretion. RESULTS: Serum HGF levels in obese subjects were more than three-fold higher than those of lean subjects (2,462 ± 184 pg/ml vs. 765 ± 48 pg/ml, p < 0.0001). The VEGF levels were not significantly elevated in obese subjects (135 ± 31 pg/ml vs. 128 ± 37 pg/ml). The HGF concentrations, but not VEGF concentrations, were significantly correlated with body mass index (BMI) (p < 0.0001, r = 0.74). The observed increases in HGF concentrations of obese subjects were not secondary to insulin resistance or hypertension. Freshly isolated human adipose cells secreted HGF. CONCLUSIONS: Our results indicate that obesity is associated with a marked increase in circulating HGF levels, which correlate linearly with BMI. Because vascular growth factors have been associated with the pathogenesis of atherosclerosis, the possible role of such humoral factors as a link between obesity and cardiovascular disease is very intriguing.

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