Obligatory action of polypeptide growth factors for the il-1-mediated prostaglandin E2 production in fibroblasts

Potential role of growth factors in modulation of tissue response to IL-1

William J. Chiou, Paul D. Bonin, Jai Pal Singh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Our studies show that in connective tissue cells, induction of PGE2 synthesis in response to IL-1 requires costimulation with platelet-derived growth factor (PDGF) or fibroblast growth factor (FGF). In cells incubated in medium containing fresh serum, IL-1 induced a dose-dependent synthesis of PGE2. However, when the cells were incubated in medium containing low serum or platelet poor plasma (lacking PDGF), IL-1 alone failed to induce PGE2 synthesis. PGE2 synthesis was restored when platelet poor plasma was supplemented with PDGF. Addition of PDGF or FGF together with IL-1 resulted in a 14-and 66-fold stimulation of PGE2 synthesis, respectively. Stimulation was dependent on the concentration of both IL-1 and the growth factor. PGE2 synthesis was also dependent on the synthesis of new proteins. In cells simultaneously treated with IL-1 and PDGF, PGE2 synthesis was initiated after a lag of 2 to 3 h, proceeded first with a rapid rate for 6 h, and then with a slower rate through 24 h. PGE2 synthesis during the latter, slower phase was greatly enhanced by pretreatment with PDGF, but not by pretreatment with IL-1. PDGF pretreatment also resulted in maintenance of 10- to 12-fold higher cell surface IL-1-binding during this phase. These data provide evidence for potentially novel interactions between PDGF and IL-1 activities, one of which is the modulation of IL-1 receptors by PDGF. Furthermore, these studies suggest that by virtue of their effect on IL-1 activities, PDGF and FGF may play additional roles in connective tissues, including an indirect role in inflammatory processes.

Original languageEnglish (US)
Pages (from-to)2155-2160
Number of pages6
JournalJournal of Immunology
Volume145
Issue number7
StatePublished - Oct 1 1990
Externally publishedYes

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Platelet-Derived Growth Factor
Interleukin-1
Dinoprostone
Intercellular Signaling Peptides and Proteins
Fibroblasts
Peptides
Fibroblast Growth Factors
Blood Platelets
Connective Tissue Cells
Interleukin-1 Receptors
Serum-Free Culture Media
Connective Tissue
Maintenance

ASJC Scopus subject areas

  • Immunology

Cite this

Obligatory action of polypeptide growth factors for the il-1-mediated prostaglandin E2 production in fibroblasts : Potential role of growth factors in modulation of tissue response to IL-1. / Chiou, William J.; Bonin, Paul D.; Singh, Jai Pal.

In: Journal of Immunology, Vol. 145, No. 7, 01.10.1990, p. 2155-2160.

Research output: Contribution to journalArticle

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abstract = "Our studies show that in connective tissue cells, induction of PGE2 synthesis in response to IL-1 requires costimulation with platelet-derived growth factor (PDGF) or fibroblast growth factor (FGF). In cells incubated in medium containing fresh serum, IL-1 induced a dose-dependent synthesis of PGE2. However, when the cells were incubated in medium containing low serum or platelet poor plasma (lacking PDGF), IL-1 alone failed to induce PGE2 synthesis. PGE2 synthesis was restored when platelet poor plasma was supplemented with PDGF. Addition of PDGF or FGF together with IL-1 resulted in a 14-and 66-fold stimulation of PGE2 synthesis, respectively. Stimulation was dependent on the concentration of both IL-1 and the growth factor. PGE2 synthesis was also dependent on the synthesis of new proteins. In cells simultaneously treated with IL-1 and PDGF, PGE2 synthesis was initiated after a lag of 2 to 3 h, proceeded first with a rapid rate for 6 h, and then with a slower rate through 24 h. PGE2 synthesis during the latter, slower phase was greatly enhanced by pretreatment with PDGF, but not by pretreatment with IL-1. PDGF pretreatment also resulted in maintenance of 10- to 12-fold higher cell surface IL-1-binding during this phase. These data provide evidence for potentially novel interactions between PDGF and IL-1 activities, one of which is the modulation of IL-1 receptors by PDGF. Furthermore, these studies suggest that by virtue of their effect on IL-1 activities, PDGF and FGF may play additional roles in connective tissues, including an indirect role in inflammatory processes.",
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