Old age causes de novo intracortical bone remodeling and porosity in mice

Marilina Piemontese, Maria Almeida, Alexander Robling, Ha Neui Kim, Jinhu Xiong, Jeff D. Thostenson, Robert S. Weinstein, Stavros C. Manolagas, Charles A. O'Brien, Robert L. Jilka

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Decreased cortical thickness and increased cortical porosity are the key anatomic changes responsible for osteoporotic fractures in elderly women and men. The cellular basis of these changes is unbalanced endosteal and intracortical osteonal remodeling by the osteoclasts and osteoblasts that comprise the basic multicellular units (BMUs). Like humans, mice lose cortical bone with age, but unlike humans, this loss occurs in the face of sex steroid sufficiency. Mice are therefore an ideal model to dissect age-specific osteoporotic mechanisms. Nevertheless, lack of evidence for endosteal or intracortical remodeling in mice has raised questions about their translational relevance. We show herein that administration of the antiosteoclastogenic cytokine osteoprotegerin to Swiss Webster mice ablated not only osteoclasts, but also endosteal bone formation, demonstrating the occurrence of BMU-based endosteal remodeling. Femoral cortical thickness decreased in aged male and female C57BL/6J mice, as well as F1 hybrids of C57BL/6J and BALB/cBy mice. This decrease was greater in C57BL/6J mice, indicating a genetic influence. Moreover, endosteal remodeling became unbalanced because of increased osteoclast and decreased osteoblast numbers. The porosity of the femoral cortex increased with age but was much higher in females of both strains. Notably, the increased cortical porosity resulted from de novo intracortical remodeling by osteon-like structures. Age-dependent cortical bone loss was associated with increased osteocyte DNA damage, cellular senescence, the senescence-associated secretory phenotype, and increased levels of RANKL. The demonstration of unbalanced endosteal and intracortical remodeling in old mice validates the relevance of this animal model to involutional osteoporosis in humans.

Original languageEnglish (US)
JournalJCI insight
Volume2
Issue number17
DOIs
StatePublished - Sep 7 2017

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Bone Remodeling
Porosity
Osteoclasts
Thigh
Osteoblasts
Inbred C57BL Mouse
Haversian System
Osteoprotegerin
Osteocytes
Osteoporotic Fractures
Cell Aging
Osteogenesis
Osteoporosis
DNA Damage
Animal Models
Steroids
Cytokines
Phenotype

Keywords

  • Aging
  • Bone Biology
  • Cellular senescence
  • Osteoporosis

Cite this

Piemontese, M., Almeida, M., Robling, A., Kim, H. N., Xiong, J., Thostenson, J. D., ... Jilka, R. L. (2017). Old age causes de novo intracortical bone remodeling and porosity in mice. JCI insight, 2(17). https://doi.org/10.1172/jci.insight.93771

Old age causes de novo intracortical bone remodeling and porosity in mice. / Piemontese, Marilina; Almeida, Maria; Robling, Alexander; Kim, Ha Neui; Xiong, Jinhu; Thostenson, Jeff D.; Weinstein, Robert S.; Manolagas, Stavros C.; O'Brien, Charles A.; Jilka, Robert L.

In: JCI insight, Vol. 2, No. 17, 07.09.2017.

Research output: Contribution to journalArticle

Piemontese, M, Almeida, M, Robling, A, Kim, HN, Xiong, J, Thostenson, JD, Weinstein, RS, Manolagas, SC, O'Brien, CA & Jilka, RL 2017, 'Old age causes de novo intracortical bone remodeling and porosity in mice', JCI insight, vol. 2, no. 17. https://doi.org/10.1172/jci.insight.93771
Piemontese, Marilina ; Almeida, Maria ; Robling, Alexander ; Kim, Ha Neui ; Xiong, Jinhu ; Thostenson, Jeff D. ; Weinstein, Robert S. ; Manolagas, Stavros C. ; O'Brien, Charles A. ; Jilka, Robert L. / Old age causes de novo intracortical bone remodeling and porosity in mice. In: JCI insight. 2017 ; Vol. 2, No. 17.
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