Olfactory neurons obtained through nasal biopsy combined with laser-capture microdissection: a potential approach to study treatment response in mental disorders

Soumya Narayan, Charlee McLean, Akira Sawa, Sandra Y. Lin, Narayan Rai, Maria Mananita S. Hipolito, Nicola Cascella, John Nurnberger, Koko Ishizuka, Evaristus A. Nwulia

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Bipolar disorder (BD) is a severe neuropsychiatric disorder with poorly understood pathophysiology and typically treated with the mood stabilizer, lithium carbonate. Animal studies as well as human genetic studies indicate that lithium affects molecular targets that are involved in neuronal growth, survival and maturation, and notably molecules involved in Wnt signaling. Given the ethical challenge to obtaining brain biopsies for investigating dynamic molecular changes associated with lithium-response in the central nervous system (CNS), one may consider the use of neurons obtained from olfactory tissues to achieve this goal.The olfactory epithelium contains olfactory receptor neurons at different stages of development and glial-like supporting cells. This provides a unique opportunity to study dynamic changes in the CNS of patients with neuropsychiatric diseases, using olfactory tissue safely obtained from nasal biopsies. To overcome the drawback posed by substantial contamination of biopsied olfactory tissue with non-neuronal cells, a novel approach to obtain enriched neuronal cell populations was developed by combining nasal biopsies with laser-capture microdissection. In this study, a system for investigating treatment-associated dynamic molecular changes in neuronal tissue was developed and validated, using a small pilot sample of BD patients recruited for the study of the molecular mechanisms of lithium treatment response.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number94
DOIs
StatePublished - Dec 4 2014

Fingerprint

Microdissection
Laser Capture Microdissection
Biopsy
Nose
Mental Disorders
Neurons
Lithium
Tissue
Lasers
Neurology
Molecular Dynamics Simulation
Bipolar Disorder
Molecular dynamics
Central Nervous System
Odorant Receptors
Olfactory Receptor Neurons
Lithium Carbonate
Olfactory Mucosa
Medical Genetics
Therapeutics

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Olfactory neurons obtained through nasal biopsy combined with laser-capture microdissection : a potential approach to study treatment response in mental disorders. / Narayan, Soumya; McLean, Charlee; Sawa, Akira; Lin, Sandra Y.; Rai, Narayan; Hipolito, Maria Mananita S.; Cascella, Nicola; Nurnberger, John; Ishizuka, Koko; Nwulia, Evaristus A.

In: Journal of visualized experiments : JoVE, No. 94, 04.12.2014.

Research output: Contribution to journalArticle

Narayan, Soumya ; McLean, Charlee ; Sawa, Akira ; Lin, Sandra Y. ; Rai, Narayan ; Hipolito, Maria Mananita S. ; Cascella, Nicola ; Nurnberger, John ; Ishizuka, Koko ; Nwulia, Evaristus A. / Olfactory neurons obtained through nasal biopsy combined with laser-capture microdissection : a potential approach to study treatment response in mental disorders. In: Journal of visualized experiments : JoVE. 2014 ; No. 94.
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