Oligonudeotide-targeted degradation of U1 and U2 snRNAs reveals differential interactions of simian virus 40 pre-mRNAs with snrnps

Zhen Qiang Pan, Hui Ge, Xin Yuan Fu, James L. Manley, Carol Prives

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have investigated the roles of U1 and U2 snRNP particles in SV40 pre-mRNA splicing by oligonucleotide-targeted degradation of U1 or U2 snRNAs in Xenopus laevis oocytes. Microinjection of oligonucleotides complementary to regions of U1 or U2 RNAs either in the presence or absence of SV40 DNA resulted in specific cleavage of the corresponding snRNA. Unexpectedly, degradation of U1 or U2 snRNA was far more extensive when the oligonucleotide was injected without, or prior to, introduction of viral DNA. In either co-injected or pre-injected oocytes, these oligonucleotides caused a dramatic reduction in the accumulation of spliced SV40 mRNA expressed from the viral late region, and a commensurate increase in unspliced late RNA. When pre-injected, two different U2 specific oligonucleotides also inhibited the formation of both large and small tumor antigen spliced early mRNAs. However, even when, by pre-injection of a U1 5′ end-specific oligonucleotide, greater than 95% degradation of the U1 snRNA 5′ ends occurred in oocytes, no reduction in early pre-mRNA splicing was observed. In contrast, the same U1 5′ end oligonucleotide, when added to HeLa splicing extracts, substantially inhibited the splicing of SV40 early pre-mRNA, indicating that U1 snRNP is not totally dispensable for early splicing. These findings confirm and extend our earlier observations which suggested that different pre-mRNAs vary in their requirements for snRNPs.

Original languageEnglish (US)
Pages (from-to)6553-6568
Number of pages16
JournalNucleic Acids Research
Volume17
Issue number16
DOIs
StatePublished - Aug 25 1989
Externally publishedYes

Fingerprint

Simian virus 40
Oligonucleotides
RNA Precursors
Viruses
Messenger RNA
Virus
Degradation
Oocyte
Interaction
U1 Small Nuclear Ribonucleoproteins
Oocytes
RNA
U2 Small Nuclear Ribonucleoproteins
DNA
Small Nuclear RNA
Viral DNA
Xenopus laevis
Microinjections
Neoplasm Antigens
Antigens

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Oligonudeotide-targeted degradation of U1 and U2 snRNAs reveals differential interactions of simian virus 40 pre-mRNAs with snrnps. / Pan, Zhen Qiang; Ge, Hui; Fu, Xin Yuan; Manley, James L.; Prives, Carol.

In: Nucleic Acids Research, Vol. 17, No. 16, 25.08.1989, p. 6553-6568.

Research output: Contribution to journalArticle

Pan, Zhen Qiang ; Ge, Hui ; Fu, Xin Yuan ; Manley, James L. ; Prives, Carol. / Oligonudeotide-targeted degradation of U1 and U2 snRNAs reveals differential interactions of simian virus 40 pre-mRNAs with snrnps. In: Nucleic Acids Research. 1989 ; Vol. 17, No. 16. pp. 6553-6568.
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