Omega-3 and omega-6 fatty acids suppress ER- and oxidative stress in cultured neurons and neuronal progenitor cells from mice lacking PPT1

Sung Jo Kim, Zhongjian Zhang, Arjun Saha, Chinmoy Sarkar, Zhenwen Zhao, Yan Xu, Anil B. Mukherjee

Research output: Contribution to journalArticle

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Abstract

Reactive oxygen species (ROS) damage brain lipids, carbohydrates, proteins, as well as DNA and may contribute to neurodegeneration. We previously reported that ER- and oxidative stress cause neuronal apoptosis in infantile neuronal ceroid lipofuscinosis (INCL), a lethal neurodegenerative storage disease, caused by palmitoyl-protein thioesterase-1 (PPT1) deficiency. Polyunsaturated fatty acids (PUFA) are essential components of cell membrane phospholipids in the brain and excessive ROS may cause oxidative damage of PUFA leading to neuronal death. Using cultured neurons and neuroprogenitor cells from mice lacking Ppt1, which mimic INCL, we demonstrate that Ppt1-deficient neurons and neuroprogenitor cells contain high levels of ROS, which may cause peroxidation of PUFA and render them incapable of providing protection against oxidative stress. We tested whether treatment of these cells with omega-3 or omega-6 PUFA protects the neurons and neuroprogenitor cells from oxidative stress and suppress apoptosis. We report here that both omega-3 and omega-6 fatty acids protect the Ppt1-deficient cells from ER- as well as oxidative stress and suppress apoptosis. Our results suggest that PUFA supplementation may have neuroprotective effects in INCL.

Original languageEnglish
Pages (from-to)292-296
Number of pages5
JournalNeuroscience Letters
Volume479
Issue number3
DOIs
StatePublished - Aug 2010

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Omega-6 Fatty Acids
Unsaturated Fatty Acids
Neuronal Ceroid-Lipofuscinoses
Oxidative Stress
Stem Cells
Neurons
Reactive Oxygen Species
Apoptosis
Brain
Neuroprotective Agents
Neurodegenerative Diseases
Phospholipids
Carbohydrates
Cell Membrane
palmitoyl-protein thioesterase
Lipids
DNA
Ceroid lipofuscinosis, neuronal 1, infantile
Proteins

Keywords

  • Apoptosis
  • Batten disease
  • ER-stress
  • INCL
  • Neurodegeneration
  • Oxidative stress
  • Palmitoyl-protein thioesterase-1
  • PUFA

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Omega-3 and omega-6 fatty acids suppress ER- and oxidative stress in cultured neurons and neuronal progenitor cells from mice lacking PPT1. / Kim, Sung Jo; Zhang, Zhongjian; Saha, Arjun; Sarkar, Chinmoy; Zhao, Zhenwen; Xu, Yan; Mukherjee, Anil B.

In: Neuroscience Letters, Vol. 479, No. 3, 08.2010, p. 292-296.

Research output: Contribution to journalArticle

Kim, Sung Jo ; Zhang, Zhongjian ; Saha, Arjun ; Sarkar, Chinmoy ; Zhao, Zhenwen ; Xu, Yan ; Mukherjee, Anil B. / Omega-3 and omega-6 fatty acids suppress ER- and oxidative stress in cultured neurons and neuronal progenitor cells from mice lacking PPT1. In: Neuroscience Letters. 2010 ; Vol. 479, No. 3. pp. 292-296.
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