On the lipolytic action of parathyroid hormone in man

Tushar K. Sinha, Payup Thajchayapong, Sherry F. Queener, Donald O. Allen, Norman H. Bell

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

An investigation was carried out to determine whether bovine PTH stimulates lipolysis in human fat tissue, whether this action is mediated by cyclic adenosine 3,5 monophosphate and whether the N terminal 1-34 peptide of bovine PTH is responsible for the lipolytic effect. Studies were also performed to determine if parathyroid extract (PTE) produces lipolysis in normal subjects and in patients with pseudohypoparathyroidism in whom there is a defect in the adenylate system in response to PTH in the renal cortex and presumably in the skeletal system as well. It was found that highly purified bovine PTH in the concentration range between 10-9 M and 10-5 M stimulated lipolysis in vitro by human fat in a dose dependent manner. Significant increases in glycerol production were observed at concentrations of PTH as low as 10-9 M and maximal increases were seen at 10-6 M. The hormone significantly increased the concentration of cyclic adenosine 3,5 monophosphate in fat tissue. The synthetic N terminal 1-34 peptide of bovine PTH was as effective as the native hormone in stimulating glycerol production at a concentration of 10-9 M 10-6 M. PTE, 100 mU per kg per min for 30 min given intravenously, produced transient increases in the concentration of plasma free fatty acid in each of eight normal subjects, three patients with hypoparathyroidism and eight patients with pseudohypoparathyroidism. Purified bovine PTH also increased plasma free fatty acid in each of two normal subjects. It is concluded that PTH stimulates lipolysis in human subcutaneous fat, that this action of the hormone is mediated through cyclic adenosine 3,5 monophosphate and that the N terminal 1-34 peptide portion of the hormone is responsible for this lipolytic action. Further, PTE stimulates lipolysis in vivo in man. There appears to be no defect in the adenylate cyclase system in the fat cell in response to PTH in patients with pseudohypoparathyroidism.

Original languageEnglish (US)
Pages (from-to)251-260
Number of pages10
JournalMetabolism
Volume25
Issue number3
DOIs
StatePublished - Mar 1976

Fingerprint

Lipolysis
Parathyroid Hormone
Pseudohypoparathyroidism
Adenosine
Fats
Hormones
Nonesterified Fatty Acids
Glycerol
Hypoparathyroidism
Peptides
Peptide Hormones
Subcutaneous Fat
Adenylyl Cyclases
Adipocytes
Kidney

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Sinha, T. K., Thajchayapong, P., Queener, S. F., Allen, D. O., & Bell, N. H. (1976). On the lipolytic action of parathyroid hormone in man. Metabolism, 25(3), 251-260. https://doi.org/10.1016/0026-0495(76)90083-4

On the lipolytic action of parathyroid hormone in man. / Sinha, Tushar K.; Thajchayapong, Payup; Queener, Sherry F.; Allen, Donald O.; Bell, Norman H.

In: Metabolism, Vol. 25, No. 3, 03.1976, p. 251-260.

Research output: Contribution to journalArticle

Sinha, Tushar K. ; Thajchayapong, Payup ; Queener, Sherry F. ; Allen, Donald O. ; Bell, Norman H. / On the lipolytic action of parathyroid hormone in man. In: Metabolism. 1976 ; Vol. 25, No. 3. pp. 251-260.
@article{5b4137a1fb584fc5bb599029f0924c8c,
title = "On the lipolytic action of parathyroid hormone in man",
abstract = "An investigation was carried out to determine whether bovine PTH stimulates lipolysis in human fat tissue, whether this action is mediated by cyclic adenosine 3,5 monophosphate and whether the N terminal 1-34 peptide of bovine PTH is responsible for the lipolytic effect. Studies were also performed to determine if parathyroid extract (PTE) produces lipolysis in normal subjects and in patients with pseudohypoparathyroidism in whom there is a defect in the adenylate system in response to PTH in the renal cortex and presumably in the skeletal system as well. It was found that highly purified bovine PTH in the concentration range between 10-9 M and 10-5 M stimulated lipolysis in vitro by human fat in a dose dependent manner. Significant increases in glycerol production were observed at concentrations of PTH as low as 10-9 M and maximal increases were seen at 10-6 M. The hormone significantly increased the concentration of cyclic adenosine 3,5 monophosphate in fat tissue. The synthetic N terminal 1-34 peptide of bovine PTH was as effective as the native hormone in stimulating glycerol production at a concentration of 10-9 M 10-6 M. PTE, 100 mU per kg per min for 30 min given intravenously, produced transient increases in the concentration of plasma free fatty acid in each of eight normal subjects, three patients with hypoparathyroidism and eight patients with pseudohypoparathyroidism. Purified bovine PTH also increased plasma free fatty acid in each of two normal subjects. It is concluded that PTH stimulates lipolysis in human subcutaneous fat, that this action of the hormone is mediated through cyclic adenosine 3,5 monophosphate and that the N terminal 1-34 peptide portion of the hormone is responsible for this lipolytic action. Further, PTE stimulates lipolysis in vivo in man. There appears to be no defect in the adenylate cyclase system in the fat cell in response to PTH in patients with pseudohypoparathyroidism.",
author = "Sinha, {Tushar K.} and Payup Thajchayapong and Queener, {Sherry F.} and Allen, {Donald O.} and Bell, {Norman H.}",
year = "1976",
month = "3",
doi = "10.1016/0026-0495(76)90083-4",
language = "English (US)",
volume = "25",
pages = "251--260",
journal = "Metabolism: Clinical and Experimental",
issn = "0026-0495",
publisher = "W.B. Saunders Ltd",
number = "3",

}

TY - JOUR

T1 - On the lipolytic action of parathyroid hormone in man

AU - Sinha, Tushar K.

AU - Thajchayapong, Payup

AU - Queener, Sherry F.

AU - Allen, Donald O.

AU - Bell, Norman H.

PY - 1976/3

Y1 - 1976/3

N2 - An investigation was carried out to determine whether bovine PTH stimulates lipolysis in human fat tissue, whether this action is mediated by cyclic adenosine 3,5 monophosphate and whether the N terminal 1-34 peptide of bovine PTH is responsible for the lipolytic effect. Studies were also performed to determine if parathyroid extract (PTE) produces lipolysis in normal subjects and in patients with pseudohypoparathyroidism in whom there is a defect in the adenylate system in response to PTH in the renal cortex and presumably in the skeletal system as well. It was found that highly purified bovine PTH in the concentration range between 10-9 M and 10-5 M stimulated lipolysis in vitro by human fat in a dose dependent manner. Significant increases in glycerol production were observed at concentrations of PTH as low as 10-9 M and maximal increases were seen at 10-6 M. The hormone significantly increased the concentration of cyclic adenosine 3,5 monophosphate in fat tissue. The synthetic N terminal 1-34 peptide of bovine PTH was as effective as the native hormone in stimulating glycerol production at a concentration of 10-9 M 10-6 M. PTE, 100 mU per kg per min for 30 min given intravenously, produced transient increases in the concentration of plasma free fatty acid in each of eight normal subjects, three patients with hypoparathyroidism and eight patients with pseudohypoparathyroidism. Purified bovine PTH also increased plasma free fatty acid in each of two normal subjects. It is concluded that PTH stimulates lipolysis in human subcutaneous fat, that this action of the hormone is mediated through cyclic adenosine 3,5 monophosphate and that the N terminal 1-34 peptide portion of the hormone is responsible for this lipolytic action. Further, PTE stimulates lipolysis in vivo in man. There appears to be no defect in the adenylate cyclase system in the fat cell in response to PTH in patients with pseudohypoparathyroidism.

AB - An investigation was carried out to determine whether bovine PTH stimulates lipolysis in human fat tissue, whether this action is mediated by cyclic adenosine 3,5 monophosphate and whether the N terminal 1-34 peptide of bovine PTH is responsible for the lipolytic effect. Studies were also performed to determine if parathyroid extract (PTE) produces lipolysis in normal subjects and in patients with pseudohypoparathyroidism in whom there is a defect in the adenylate system in response to PTH in the renal cortex and presumably in the skeletal system as well. It was found that highly purified bovine PTH in the concentration range between 10-9 M and 10-5 M stimulated lipolysis in vitro by human fat in a dose dependent manner. Significant increases in glycerol production were observed at concentrations of PTH as low as 10-9 M and maximal increases were seen at 10-6 M. The hormone significantly increased the concentration of cyclic adenosine 3,5 monophosphate in fat tissue. The synthetic N terminal 1-34 peptide of bovine PTH was as effective as the native hormone in stimulating glycerol production at a concentration of 10-9 M 10-6 M. PTE, 100 mU per kg per min for 30 min given intravenously, produced transient increases in the concentration of plasma free fatty acid in each of eight normal subjects, three patients with hypoparathyroidism and eight patients with pseudohypoparathyroidism. Purified bovine PTH also increased plasma free fatty acid in each of two normal subjects. It is concluded that PTH stimulates lipolysis in human subcutaneous fat, that this action of the hormone is mediated through cyclic adenosine 3,5 monophosphate and that the N terminal 1-34 peptide portion of the hormone is responsible for this lipolytic action. Further, PTE stimulates lipolysis in vivo in man. There appears to be no defect in the adenylate cyclase system in the fat cell in response to PTH in patients with pseudohypoparathyroidism.

UR - http://www.scopus.com/inward/record.url?scp=84886623469&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886623469&partnerID=8YFLogxK

U2 - 10.1016/0026-0495(76)90083-4

DO - 10.1016/0026-0495(76)90083-4

M3 - Article

C2 - 175239

AN - SCOPUS:84886623469

VL - 25

SP - 251

EP - 260

JO - Metabolism: Clinical and Experimental

JF - Metabolism: Clinical and Experimental

SN - 0026-0495

IS - 3

ER -