Optical methods in renal research

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Light microscopy is a powerful experimental approach that allows the researcher to study the dynamics of cellular responses and subcellular organization. Recent advances in optics, detectors, computerized image processing, and the development of vital dyes promise to extend the utility of light microscopy. Quantitative approaches measuring intracellular pH, sodium or calcium are well known, but future developments may permit quantitative analysis of immunolabeled specimens. This review will describe the basic features of light microscopes and imaging techniques. Specific types of microscopy such as confocal microscopy, wide field microscopes, and epifluorescence microscopy will be considered. In addition, recent advances in cellular labeling techniques will be described. Post production image processing techniques and computerized deconvolution methods will be discussed, and lastly future developments in optics, improved sensitivity in detectors with enhanced signal-to-noise ratios will be explored. Wherever possible, specific applications that have been applied to renal physiology studies will be cited.

Original languageEnglish
Pages (from-to)122-137
Number of pages16
JournalSeminars in Nephrology
Volume18
Issue number2
StatePublished - 1998

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Microscopy
Kidney
Research
Light
Signal-To-Noise Ratio
Confocal Microscopy
Coloring Agents
Sodium
Research Personnel
Calcium

ASJC Scopus subject areas

  • Nephrology

Cite this

Optical methods in renal research. / Dunn, Kenneth; Bacallao, Robert.

In: Seminars in Nephrology, Vol. 18, No. 2, 1998, p. 122-137.

Research output: Contribution to journalArticle

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