Optical recording of single cardiomyocyte transmembrane potential in langendorff-perfused mouse hearts

G. Bu, E. J. Berbari, M. Rubart

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Spatial heterogeneity of action potential properties has been related to cardiac arrhythmogenesis. In this study we used laser scanning confocal microscopy in conjunction with the fast potentiometric dye ANNINE-6 to monitor changes in cardiomyocyte transmembrane potentials in Langendorff-perfused mouse hearts on a subcellular scale. Line-scan images from up to three neighboring cardiomyocytes were obtained during continuous electrical stimulation at 3 Hz. Fluorescence changes for each cardiomyocyte along the scan line were resolved from the corresponding line-scan image. Peak changes in fluorescence intensity during an action potential exceeded 20%. Signal-to-noise ratio of the optical signal was >20. Action potential durations were not significantly different between adjacent cardio- myocytes under our conditions. We conclude that this imaging technique can be used to investigate cell-to-cell repolarization heterogeneity in the intact heart.

Original languageEnglish (US)
Title of host publicationComputers in Cardiology 2007, CAR 2007
Pages357-360
Number of pages4
DOIs
StatePublished - Dec 1 2007
EventComputers in Cardiology 2007, CAR 2007 - Durham, NC, United States
Duration: Sep 30 2007Oct 3 2007

Publication series

NameComputers in Cardiology
Volume34
ISSN (Print)0276-6574

Other

OtherComputers in Cardiology 2007, CAR 2007
CountryUnited States
CityDurham, NC
Period9/30/0710/3/07

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ASJC Scopus subject areas

  • Computer Science Applications
  • Cardiology and Cardiovascular Medicine

Cite this

Bu, G., Berbari, E. J., & Rubart, M. (2007). Optical recording of single cardiomyocyte transmembrane potential in langendorff-perfused mouse hearts. In Computers in Cardiology 2007, CAR 2007 (pp. 357-360). [4745495] (Computers in Cardiology; Vol. 34). https://doi.org/10.1109/CIC.2007.4745495