Optical recording of single cardiomyocyte transmembrane potential in langendorff-perfused mouse hearts

G. Bu, E. J. Berbari, Michael Rubart-von der Lohe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Spatial heterogeneity of action potential properties has been related to cardiac arrhythmogenesis. In this study we used laser scanning confocal microscopy in conjunction with the fast potentiometric dye ANNINE-6 to monitor changes in cardiomyocyte transmembrane potentials in Langendorff-perfused mouse hearts on a subcellular scale. Line-scan images from up to three neighboring cardiomyocytes were obtained during continuous electrical stimulation at 3 Hz. Fluorescence changes for each cardiomyocyte along the scan line were resolved from the corresponding line-scan image. Peak changes in fluorescence intensity during an action potential exceeded 20%. Signal-to-noise ratio of the optical signal was >20. Action potential durations were not significantly different between adjacent cardio- myocytes under our conditions. We conclude that this imaging technique can be used to investigate cell-to-cell repolarization heterogeneity in the intact heart.

Original languageEnglish
Title of host publicationComputers in Cardiology
Pages357-360
Number of pages4
Volume34
DOIs
StatePublished - 2007
EventComputers in Cardiology 2007, CAR 2007 - Durham, NC, United States
Duration: Sep 30 2007Oct 3 2007

Other

OtherComputers in Cardiology 2007, CAR 2007
CountryUnited States
CityDurham, NC
Period9/30/0710/3/07

Fingerprint

Optical recording
Cardiac Myocytes
Membrane Potentials
Action Potentials
Fluorescence
Confocal microscopy
Signal to noise ratio
Dyes
Signal-To-Noise Ratio
Scanning
Imaging techniques
Confocal Microscopy
Muscle Cells
Electric Stimulation
Lasers
Coloring Agents
Isolated Heart Preparation

ASJC Scopus subject areas

  • Computer Science Applications
  • Cardiology and Cardiovascular Medicine

Cite this

Optical recording of single cardiomyocyte transmembrane potential in langendorff-perfused mouse hearts. / Bu, G.; Berbari, E. J.; Rubart-von der Lohe, Michael.

Computers in Cardiology. Vol. 34 2007. p. 357-360 4745495.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bu, G, Berbari, EJ & Rubart-von der Lohe, M 2007, Optical recording of single cardiomyocyte transmembrane potential in langendorff-perfused mouse hearts. in Computers in Cardiology. vol. 34, 4745495, pp. 357-360, Computers in Cardiology 2007, CAR 2007, Durham, NC, United States, 9/30/07. https://doi.org/10.1109/CIC.2007.4745495
@inproceedings{d3ee37ab9758412da48bbdb3582ed316,
title = "Optical recording of single cardiomyocyte transmembrane potential in langendorff-perfused mouse hearts",
abstract = "Spatial heterogeneity of action potential properties has been related to cardiac arrhythmogenesis. In this study we used laser scanning confocal microscopy in conjunction with the fast potentiometric dye ANNINE-6 to monitor changes in cardiomyocyte transmembrane potentials in Langendorff-perfused mouse hearts on a subcellular scale. Line-scan images from up to three neighboring cardiomyocytes were obtained during continuous electrical stimulation at 3 Hz. Fluorescence changes for each cardiomyocyte along the scan line were resolved from the corresponding line-scan image. Peak changes in fluorescence intensity during an action potential exceeded 20{\%}. Signal-to-noise ratio of the optical signal was >20. Action potential durations were not significantly different between adjacent cardio- myocytes under our conditions. We conclude that this imaging technique can be used to investigate cell-to-cell repolarization heterogeneity in the intact heart.",
author = "G. Bu and Berbari, {E. J.} and {Rubart-von der Lohe}, Michael",
year = "2007",
doi = "10.1109/CIC.2007.4745495",
language = "English",
isbn = "9781424425334",
volume = "34",
pages = "357--360",
booktitle = "Computers in Cardiology",

}

TY - GEN

T1 - Optical recording of single cardiomyocyte transmembrane potential in langendorff-perfused mouse hearts

AU - Bu, G.

AU - Berbari, E. J.

AU - Rubart-von der Lohe, Michael

PY - 2007

Y1 - 2007

N2 - Spatial heterogeneity of action potential properties has been related to cardiac arrhythmogenesis. In this study we used laser scanning confocal microscopy in conjunction with the fast potentiometric dye ANNINE-6 to monitor changes in cardiomyocyte transmembrane potentials in Langendorff-perfused mouse hearts on a subcellular scale. Line-scan images from up to three neighboring cardiomyocytes were obtained during continuous electrical stimulation at 3 Hz. Fluorescence changes for each cardiomyocyte along the scan line were resolved from the corresponding line-scan image. Peak changes in fluorescence intensity during an action potential exceeded 20%. Signal-to-noise ratio of the optical signal was >20. Action potential durations were not significantly different between adjacent cardio- myocytes under our conditions. We conclude that this imaging technique can be used to investigate cell-to-cell repolarization heterogeneity in the intact heart.

AB - Spatial heterogeneity of action potential properties has been related to cardiac arrhythmogenesis. In this study we used laser scanning confocal microscopy in conjunction with the fast potentiometric dye ANNINE-6 to monitor changes in cardiomyocyte transmembrane potentials in Langendorff-perfused mouse hearts on a subcellular scale. Line-scan images from up to three neighboring cardiomyocytes were obtained during continuous electrical stimulation at 3 Hz. Fluorescence changes for each cardiomyocyte along the scan line were resolved from the corresponding line-scan image. Peak changes in fluorescence intensity during an action potential exceeded 20%. Signal-to-noise ratio of the optical signal was >20. Action potential durations were not significantly different between adjacent cardio- myocytes under our conditions. We conclude that this imaging technique can be used to investigate cell-to-cell repolarization heterogeneity in the intact heart.

UR - http://www.scopus.com/inward/record.url?scp=62949102964&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62949102964&partnerID=8YFLogxK

U2 - 10.1109/CIC.2007.4745495

DO - 10.1109/CIC.2007.4745495

M3 - Conference contribution

AN - SCOPUS:62949102964

SN - 9781424425334

VL - 34

SP - 357

EP - 360

BT - Computers in Cardiology

ER -