Optimization of autologous muscle stem cell survival in the denervated hemilarynx

Stacey L. Halum, Kelly K. Hiatt, Moumita Naidu, Ahmed S. Sufyan, D. Wade Clapp

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Objective: Current treatments for vocal fold paralysis are suboptimal in that they fail to restore dynamic function. Autologous muscle stem cell (MSC) therapy is a promising potential therapy for vocal fold paralysis in that it can attenuate denervation-induced muscle atrophy and provide a vehicle for delivery of neurotrophic factors, thereby potentially selectively guiding reinnervation. The goal of this project was to characterize optimal conditions for injected autologous MSC survival in the thyroarytenoid (TA) muscle following recurrent laryngeal nerve (RLN) injury by local administration of adjuvant factors. Study Design: Animal experiment. Methods: Unilateral RLN transection and sternocleidomastoid muscle (̃ 1 g) biopsies were performed in 20 male Wis tar rats. One month later, 106 autologous MSCs labeled via retro viral-enhanced green fluorescent protein (EGFP) transduction were injected into the denervated hemilarynx of each animal with one of four adjuvant therapies: cardiotoxin [(CTX) 10-5 M], insulinlike growth factor-1 [(IGF- 1) 100 μg/mL], ciliary neurotrophic factor [(CNTF) 50 μg/mL], or saline. Animals were euthanized 1 month later and larynges harvested, sectioned, and analyzed for MSC survival. Results: All specimens demonstrate extensive MSC survival, with fusion of the MSCs with the denervated myofibers. Based on mean fluorescent intensity of the laryngeal specimens, IGF-1 and CNTF had the greatest positive influence on MSC survival. Myofiber diameters demonstrated myofiber atrophy to be inversely related to MSC survival, with the least atrophy in the groups having the greatest MSC survival. Conclusions: Autologous MSC therapy may be a future treatment for vocal fold paralysis. These findings support a model whereby MSCs genetically engineered to secrete CNTF and/or IGF-1 may not only promote neural regeneration, but also enhance MSC survival in an autocrine fashion.

Original languageEnglish (US)
Pages (from-to)1308-1312
Number of pages5
JournalLaryngoscope
Volume118
Issue number7
DOIs
StatePublished - Jul 1 2008

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Keywords

  • Atrophy
  • Ciliary-derived neurotrophic factor
  • CNTF
  • IGF-1
  • Insulin-like growth factor-1
  • Laryngeal denervation
  • Laryngeal paralysis
  • Muscle stem cells
  • Myoblasts
  • Myofiber
  • Neurotrophic factors
  • Satellite cell
  • Vocal cord paralysis
  • Vocal fold paralysis

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Medicine(all)

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