Optimization of immunochemical methods for intracellular protein detection.

Daniela Petrusca, O. M. Popa, L. Gavrilǎ, A. Sulicǎ

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Given the possibility that cell kinetics and p53 status may be important determinants of chemotherapeutical efficacy, the aim of the present study was to determine the optimal methods and conditions for qualitative and quantitative intracellular proteins detection. Bradford assay is the better choice for protein concentration detection because it is more sensitive and more rapid than Sheffield assay despite the fact that it utilizes a higher amount of samples. The direct staining method for flow-cytometrical detection of intracellular proteins is more rapid as compared to the indirect staining one, also providing information about protein expression during cell cycle phases, but it is low sensitive for protein expression estimation and is prohibitive for masked intracellular proteins like PCNA. More than that, it can be performed with both fixed and freshly isolated cells as compared to the indirect staining method, but the last one provides advantages by signal amplification and by its availability of using it for a large number of intracellular proteins detection.

Original languageEnglish (US)
Title of host publicationRomanian journal of virology
Pages33-41
Number of pages9
Volume50
Edition1-4
StatePublished - Jan 1999
Externally publishedYes

Fingerprint

Proteins
Staining and Labeling
Proliferating Cell Nuclear Antigen
Cell Cycle

ASJC Scopus subject areas

  • Virology

Cite this

Petrusca, D., Popa, O. M., Gavrilǎ, L., & Sulicǎ, A. (1999). Optimization of immunochemical methods for intracellular protein detection. In Romanian journal of virology (1-4 ed., Vol. 50, pp. 33-41)

Optimization of immunochemical methods for intracellular protein detection. / Petrusca, Daniela; Popa, O. M.; Gavrilǎ, L.; Sulicǎ, A.

Romanian journal of virology. Vol. 50 1-4. ed. 1999. p. 33-41.

Research output: Chapter in Book/Report/Conference proceedingChapter

Petrusca, D, Popa, OM, Gavrilǎ, L & Sulicǎ, A 1999, Optimization of immunochemical methods for intracellular protein detection. in Romanian journal of virology. 1-4 edn, vol. 50, pp. 33-41.
Petrusca D, Popa OM, Gavrilǎ L, Sulicǎ A. Optimization of immunochemical methods for intracellular protein detection. In Romanian journal of virology. 1-4 ed. Vol. 50. 1999. p. 33-41
Petrusca, Daniela ; Popa, O. M. ; Gavrilǎ, L. ; Sulicǎ, A. / Optimization of immunochemical methods for intracellular protein detection. Romanian journal of virology. Vol. 50 1-4. ed. 1999. pp. 33-41
@inbook{76b2011802554b25860a6825b33377d1,
title = "Optimization of immunochemical methods for intracellular protein detection.",
abstract = "Given the possibility that cell kinetics and p53 status may be important determinants of chemotherapeutical efficacy, the aim of the present study was to determine the optimal methods and conditions for qualitative and quantitative intracellular proteins detection. Bradford assay is the better choice for protein concentration detection because it is more sensitive and more rapid than Sheffield assay despite the fact that it utilizes a higher amount of samples. The direct staining method for flow-cytometrical detection of intracellular proteins is more rapid as compared to the indirect staining one, also providing information about protein expression during cell cycle phases, but it is low sensitive for protein expression estimation and is prohibitive for masked intracellular proteins like PCNA. More than that, it can be performed with both fixed and freshly isolated cells as compared to the indirect staining method, but the last one provides advantages by signal amplification and by its availability of using it for a large number of intracellular proteins detection.",
author = "Daniela Petrusca and Popa, {O. M.} and L. Gavrilǎ and A. Sulicǎ",
year = "1999",
month = "1",
language = "English (US)",
volume = "50",
pages = "33--41",
booktitle = "Romanian journal of virology",
edition = "1-4",

}

TY - CHAP

T1 - Optimization of immunochemical methods for intracellular protein detection.

AU - Petrusca, Daniela

AU - Popa, O. M.

AU - Gavrilǎ, L.

AU - Sulicǎ, A.

PY - 1999/1

Y1 - 1999/1

N2 - Given the possibility that cell kinetics and p53 status may be important determinants of chemotherapeutical efficacy, the aim of the present study was to determine the optimal methods and conditions for qualitative and quantitative intracellular proteins detection. Bradford assay is the better choice for protein concentration detection because it is more sensitive and more rapid than Sheffield assay despite the fact that it utilizes a higher amount of samples. The direct staining method for flow-cytometrical detection of intracellular proteins is more rapid as compared to the indirect staining one, also providing information about protein expression during cell cycle phases, but it is low sensitive for protein expression estimation and is prohibitive for masked intracellular proteins like PCNA. More than that, it can be performed with both fixed and freshly isolated cells as compared to the indirect staining method, but the last one provides advantages by signal amplification and by its availability of using it for a large number of intracellular proteins detection.

AB - Given the possibility that cell kinetics and p53 status may be important determinants of chemotherapeutical efficacy, the aim of the present study was to determine the optimal methods and conditions for qualitative and quantitative intracellular proteins detection. Bradford assay is the better choice for protein concentration detection because it is more sensitive and more rapid than Sheffield assay despite the fact that it utilizes a higher amount of samples. The direct staining method for flow-cytometrical detection of intracellular proteins is more rapid as compared to the indirect staining one, also providing information about protein expression during cell cycle phases, but it is low sensitive for protein expression estimation and is prohibitive for masked intracellular proteins like PCNA. More than that, it can be performed with both fixed and freshly isolated cells as compared to the indirect staining method, but the last one provides advantages by signal amplification and by its availability of using it for a large number of intracellular proteins detection.

UR - http://www.scopus.com/inward/record.url?scp=0032601041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032601041&partnerID=8YFLogxK

M3 - Chapter

C2 - 11601378

AN - SCOPUS:0032601041

VL - 50

SP - 33

EP - 41

BT - Romanian journal of virology

ER -