Optogenetic field potential recording in cortical slices

Wenhui Xiong, Xiaoming Jin

Research output: Contribution to journalArticle

8 Scopus citations


We introduce a method that uses optogenetic stimulation to evoke field potentials in brain slices prepared from transgenic mice expressing channelrhodopsin-2-YFP. Cortical slices in a recording chamber were stimulated with a 473. nm blue laser via either a laser scanning photostimulation setup or by direct guidance of a fiber optic. Field potentials evoked by either of the two optogenetic stimulation methods had stable amplitude, consistent waveform, and similar components as events evoked with a conventional stimulating electrode. The amplitude of evoked excitatory postsynaptic potentials increased with increasing laser intensity or pulse duration. We further demonstrated that optogenetic stimulation can be used for the induction and monitoring of long-term depression. We conclude that this technique allows for efficient and reliable activation of field potentials in brain slice preparation, and will be useful for studying short and long term synaptic plasticity.

Original languageEnglish (US)
Pages (from-to)119-124
Number of pages6
JournalJournal of Neuroscience Methods
Issue number2
StatePublished - Sep 30 2012


  • Brain slice
  • Cerebral cortex
  • Channelrhodopsin-2
  • Field potential recording
  • Long-term depression
  • Optogenetics

ASJC Scopus subject areas

  • Neuroscience(all)

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