Oral lichen planus: An immunohistochemical study of heat shock proteins (HSPS) and cytokeratins (CKS) and a unifying hypothesis of pathogenesis

Ponlatham Chaiyarit, Abdel H. Kafrawy, Dale A. Miles, Susan L. Zunt, Margot L. Van Dis, Richard L. Gregory

Research output: Contribution to journalArticle

50 Scopus citations


The expression of heat shock proteins HSP60 and HSP70 and cytokeratins CK1/10 and CK7/18 were compared in epithelium of oral lichen planus (OLP) lesions and oral fibromas using an avidin-biotin-peroxidase complex (ABC) immunohistochemical method. An immunostaining intensity distribution (IID) index was developed to assess staining intensity and the proportion of positively stained cells in different layers of the epithelium. The expression of HSP60 in the basal layer was significantly higher in OLP than in fibromas. No difference in HSP70 expression was evident between OLP and fibromas. The expression of CK1/10 in the epithelial basal and suprabasal layers was significantly higher in OLP than in fibromas. There was no demonstrable staining for CK7/18 in either OLP or fibromas. A significant correlation was evident between the expression of HSP60 and CK1/10 in the basal epithelial cells in OLP. The findings support a role for HSP60 in the pathogenesis of OLP. A unifying hypothesis of the pathogenesis of OLP, involving two sequential immune reactions, is proposed.

Original languageEnglish (US)
Pages (from-to)210-215
Number of pages6
JournalJournal of Oral Pathology and Medicine
Issue number5
StatePublished - May 1 1999



  • Avidin-biotin-peroxidase complex (ABC)
  • Cytokeratins (CKs)
  • Heat shock proteins (HSP)
  • Immunostaining intensity distribution (IID)
  • Oral lichen planus (OLP)

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Dentistry(all)

Cite this