Organ-specific (localized) synthesis of Ig light chain amyloid

Kamran Hamidi Asl, Juris J. Liepnieks, Masaaki Nakamura, Merrill Benson

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Ig amyloidosis is usually a systemic disease with multisystem involvement. However, in a significant number of cases amyloid deposition is limited to one specific organ. It has not been determined if the Ig light chain (LC) amyloid precursor protein in localized amyloidosis is synthesized by circulating plasma cells with targeting of the amyloid fibril-forming process to one specific organ, or whether the synthesis of Ig LC and fibril formation occurs entirely as a localized process. In the present study local synthesis of an amyloid fibril precursor LC was investigated. Amyloid fibrils were isolated from a ureter that was obstructed by extensive infiltration of the wall with amyloid. Amino acid sequence analysis of the isolated fibril subunit protein proved it to be derived from a λ(II) Ig LC. Plasma cells within the lesion stained positively with labeled anti-λ Ab and by in situ hybridization using an oligonucleotide probe specific for λ-LC mRNA. RT-PCR of mRNA extracted from the tumor and direct DNA sequencing gave the nucleotide sequence coding specifically for the λ(II) amyloid subunit protein, thus confirming local synthesis of the LC protein.

Original languageEnglish
Pages (from-to)5556-5560
Number of pages5
JournalJournal of Immunology
Volume162
Issue number9
StatePublished - May 1 1999

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Amyloid
Light
Amyloidosis
Plasma Cells
Amyloidogenic Proteins
Messenger RNA
Oligonucleotide Probes
Amyloid beta-Protein Precursor
Protein Sequence Analysis
Protein Subunits
Ureter
DNA Sequence Analysis
In Situ Hybridization
Polymerase Chain Reaction
Neoplasms
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Hamidi Asl, K., Liepnieks, J. J., Nakamura, M., & Benson, M. (1999). Organ-specific (localized) synthesis of Ig light chain amyloid. Journal of Immunology, 162(9), 5556-5560.

Organ-specific (localized) synthesis of Ig light chain amyloid. / Hamidi Asl, Kamran; Liepnieks, Juris J.; Nakamura, Masaaki; Benson, Merrill.

In: Journal of Immunology, Vol. 162, No. 9, 01.05.1999, p. 5556-5560.

Research output: Contribution to journalArticle

Hamidi Asl, K, Liepnieks, JJ, Nakamura, M & Benson, M 1999, 'Organ-specific (localized) synthesis of Ig light chain amyloid', Journal of Immunology, vol. 162, no. 9, pp. 5556-5560.
Hamidi Asl K, Liepnieks JJ, Nakamura M, Benson M. Organ-specific (localized) synthesis of Ig light chain amyloid. Journal of Immunology. 1999 May 1;162(9):5556-5560.
Hamidi Asl, Kamran ; Liepnieks, Juris J. ; Nakamura, Masaaki ; Benson, Merrill. / Organ-specific (localized) synthesis of Ig light chain amyloid. In: Journal of Immunology. 1999 ; Vol. 162, No. 9. pp. 5556-5560.
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