Organic cation transport in the rat kidney in vivo visualized by time-resolved two-photon microscopy

M. Hörbelt, C. Wotzlaw, Timothy Sutton, Bruce Molitoris, T. Philipp, A. Kribben, J. Fandrey, F. Pietruck

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Secretion of cationic drugs and endogenous metabolites is a major function of the kidney accomplished by tubular organic cation transport systems. A cationic styryl dye (ASP+) was developed as a fluorescent substrate for renal organic cation transporters. The dye was injected intravenously and continuously monitored in externalized rat kidneys by time-resolved two-photon laser scanning microscopy. To investigate changes in transport activity, cimetidine, a competitive inhibitor of organic cation transport was co-injected with ASP+. Shortly after injection, fluorescence increased in peritubular capillaries. Simultaneously, fluorescence was transiently found at the basolateral membrane of the proximal and distal tubules at a higher intensity and shorter wavelength indicating membrane association of ASP +. Subsequently, intracellular fluorescence increased steeply within 10 s. In the proximal tubules, intracellular fluorescence decreased by 50% within 5 min, while in the distal tubules the fluorescence decreased by only 5% within the same time frame. Intracellular uptake of ASP+ into proximal tubules was significantly reduced by cimetidine. Our studies show that organic cation transport of the kidney can be visualized in vivo by two-photon laser scanning microscopy.

Original languageEnglish
Pages (from-to)422-429
Number of pages8
JournalKidney International
Volume72
Issue number4
DOIs
StatePublished - Aug 2007

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Photons
Cations
Microscopy
Fluorescence
Kidney
Cimetidine
Confocal Microscopy
Coloring Agents
Membranes
Injections
Pharmaceutical Preparations

Keywords

  • ASP
  • Drug secretion
  • Fluorescence imaging
  • Organic cation transport
  • Two-photon microscopy

ASJC Scopus subject areas

  • Nephrology

Cite this

Organic cation transport in the rat kidney in vivo visualized by time-resolved two-photon microscopy. / Hörbelt, M.; Wotzlaw, C.; Sutton, Timothy; Molitoris, Bruce; Philipp, T.; Kribben, A.; Fandrey, J.; Pietruck, F.

In: Kidney International, Vol. 72, No. 4, 08.2007, p. 422-429.

Research output: Contribution to journalArticle

Hörbelt, M. ; Wotzlaw, C. ; Sutton, Timothy ; Molitoris, Bruce ; Philipp, T. ; Kribben, A. ; Fandrey, J. ; Pietruck, F. / Organic cation transport in the rat kidney in vivo visualized by time-resolved two-photon microscopy. In: Kidney International. 2007 ; Vol. 72, No. 4. pp. 422-429.
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