Origin of guanine nucleotides in isolated heart mitochondria

Edward E. McKee, Alice T. Bentley, Ronald M. Smith, Christina E. Ciaccio

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Presence of guanine nucleotide within the matrix of mitochondria is uncontested; the mechanism by which GTP takes up residence in the matrix is unknown. In this report, we demonstrate for the first time that direct transport of guanine nucleotide across the inner membrane of heart mitochondria is possible. Transport of guanine nucleotides from the medium to the matrix was suggested by inhibition of translation in isolated rat heart mitochondria when GTP-γ-S was added to the medium. This result suggested that GTP was one source of matrix GTP. Other sources were investigated by measuring matrix uptake and conversion to GTP of several purines, purine nucleosides, and purine nucleotides. Results demonstrated that [14C]-guanine and [3H]-guanosine were not taken up by isolated mitochondria and were not converted to any other compound. While [14C]-ATP and [3H]-AMP were taken up readily into the matrix, radioactivity was never associated with a guanine compound. [3H]-IMP was not taken up into the matrix and was never converted to another compound. Our data showed that label added as [3H]-GTP, [3H]-GDP, or [3H]-GMP was readily taken up and concentrated in the matrix of isolated mitochondria.

Original languageEnglish (US)
Pages (from-to)466-472
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume257
Issue number2
DOIs
StatePublished - Apr 13 1999
Externally publishedYes

Fingerprint

Heart Mitochondria
Mitochondria
Guanine Nucleotides
Guanosine Triphosphate
Guanine
Purine Nucleosides
Purine Nucleotides
Inosine Monophosphate
Purines
Guanosine
Radioactivity
Adenosine Monophosphate
Rats
Labels
Adenosine Triphosphate
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Origin of guanine nucleotides in isolated heart mitochondria. / McKee, Edward E.; Bentley, Alice T.; Smith, Ronald M.; Ciaccio, Christina E.

In: Biochemical and Biophysical Research Communications, Vol. 257, No. 2, 13.04.1999, p. 466-472.

Research output: Contribution to journalArticle

McKee, Edward E. ; Bentley, Alice T. ; Smith, Ronald M. ; Ciaccio, Christina E. / Origin of guanine nucleotides in isolated heart mitochondria. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 257, No. 2. pp. 466-472.
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