Origins of the renal innervation in the primate, macaca fascicularis

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The origins of the renal efferent and afferent nerves in 5 cynomolgus monkeys (Macaca fascicularis) were studied by using the retrograde transport of horseradish peroxidase (HRP) and horseradish peroxidase-wheat germ agglutinin (HRP WGA). The cut ends of the right renal nerves were soaked for 30-45 min in solutions consisting of 15% HRP and 1% HRP WGA. Three or four days later the animals were killed and the tissues examined for the presence of retrogradely labeled neurons, HRP-filled cells were observed, with rare exceptions, only in ganglia ipsilateral to the side of tracer application. Renal efferent neurons (4648-14565 cells per animal) were found in relatively equal numbers in prevertebral and paravertebral (sympathetic chain) ganglia. Labeled prevertebral cells were distributed among the renal (52%), aorticorenal (32%) and superior mesenteric (16%) ganglia, whereas labeled paravertebral neurons were mainly located in chain ganglia T11-L3, with 94% of these located in L1-3.Labeled renal sensory neurons (31-543 per animal) constituted less than 5% of all labeled cells and were found in ipsilateral dorsal root ganglia T10-L3, with (80%) in T12 and L1. The labeled sensory neurons ranged from 18-64 μm in diameter (X = 32.4 μm). With the exception of a single cell in one animal, no labeled neurons were observed in the nodose ganglia. Many parallels were observed between the organization of the renal plexuses of macaques and humans, suggesting the utility of the non-human primate as an experimental model for functional studies of renal innervation in humans.

Original languageEnglish
Pages (from-to)113-126
Number of pages14
JournalJournal of the Autonomic Nervous System
Volume27
Issue number2
DOIs
StatePublished - 1989

Fingerprint

Macaca fascicularis
Primates
Horseradish Peroxidase
Kidney
Ganglia
Sensory Receptor Cells
Neurons
Efferent Neurons
Nodose Ganglion
Sympathetic Ganglia
Wheat Germ Agglutinins
Spinal Ganglia
Macaca
Theoretical Models

Keywords

  • Coeliac ganglion
  • Horseradish peroxidase
  • Mesenteric ganglion
  • Primate
  • Renal afferent nerve
  • Renal innervation
  • Sympathetic nerve

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Origins of the renal innervation in the primate, macaca fascicularis. / Marfurt, Carl; Echtenkamp, Steve; Jones, Mark A.

In: Journal of the Autonomic Nervous System, Vol. 27, No. 2, 1989, p. 113-126.

Research output: Contribution to journalArticle

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abstract = "The origins of the renal efferent and afferent nerves in 5 cynomolgus monkeys (Macaca fascicularis) were studied by using the retrograde transport of horseradish peroxidase (HRP) and horseradish peroxidase-wheat germ agglutinin (HRP WGA). The cut ends of the right renal nerves were soaked for 30-45 min in solutions consisting of 15{\%} HRP and 1{\%} HRP WGA. Three or four days later the animals were killed and the tissues examined for the presence of retrogradely labeled neurons, HRP-filled cells were observed, with rare exceptions, only in ganglia ipsilateral to the side of tracer application. Renal efferent neurons (4648-14565 cells per animal) were found in relatively equal numbers in prevertebral and paravertebral (sympathetic chain) ganglia. Labeled prevertebral cells were distributed among the renal (52{\%}), aorticorenal (32{\%}) and superior mesenteric (16{\%}) ganglia, whereas labeled paravertebral neurons were mainly located in chain ganglia T11-L3, with 94{\%} of these located in L1-3.Labeled renal sensory neurons (31-543 per animal) constituted less than 5{\%} of all labeled cells and were found in ipsilateral dorsal root ganglia T10-L3, with (80{\%}) in T12 and L1. The labeled sensory neurons ranged from 18-64 μm in diameter (X = 32.4 μm). With the exception of a single cell in one animal, no labeled neurons were observed in the nodose ganglia. Many parallels were observed between the organization of the renal plexuses of macaques and humans, suggesting the utility of the non-human primate as an experimental model for functional studies of renal innervation in humans.",
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