Orthodontic tooth movement causes decreased promoter expression of collagen type 1, bone sialoprotein and alpha-smooth muscle actin in the periodontal ligament

C. Olson, F. Uribe, Z. Kalajzic, Achint Utreja, R. Nanda, D. Rowe, S. Wadhwa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective- To evaluate the effects of orthodontic tooth movement on the promoter expression of collagen type 1 (3.6Col1), bone sialoprotein (BSP) and alpha-smooth muscle actin (αSMA) in the periodontal ligament (PDL) using transgenic mice containing transgenes of these promoters fused to green fluorescent proteins (GFP). Materials and Methods- The maxillary first molars of 10-12 week-old transgenic mice were loaded with 10-12g of force for 12, 48h, or 7days. Mice were transgenic for one of the following GFP-tagged bone markers of osteoblast lineage cells: 3.6-kb fragment of the rat collagen type 1 promoter (3.6Col1), BSP or α-smooth muscle actin (αSMA). Loaded molars under compression and tension were compared with contra-lateral unloaded controls. Results- On the compression side of the PDL, orthodontic tooth movement caused a significant decrease in GFP expression of all the promoters at each time point. On the tension side, there was a significant increase in BSP-GFP expression, 12h following loading compared to the contralateral unloaded controls. Conclusions- An in vivo tooth movement model using transgenic mice with promoter-GFP constructs provides an efficient and effective way of investigating the cellular events underlying orthodontic tooth movement. PDL cells may undergo decreased differentiation in response to the compressive force.

Original languageEnglish (US)
Pages (from-to)52-61
Number of pages10
JournalOrthodontics and Craniofacial Research
Volume15
Issue number1
DOIs
StatePublished - Feb 2012
Externally publishedYes

Fingerprint

Integrin-Binding Sialoprotein
Tooth Movement Techniques
Periodontal Ligament
Collagen Type I
Green Fluorescent Proteins
Smooth Muscle
Actins
Transgenic Mice
Osteoblasts
Transgenes
Bone and Bones

Keywords

  • Green fluorescent proteins
  • Osteoblast
  • Periodontal ligament
  • Tooth movement
  • Transgenic mice

ASJC Scopus subject areas

  • Orthodontics
  • Oral Surgery
  • Otorhinolaryngology
  • Surgery

Cite this

Orthodontic tooth movement causes decreased promoter expression of collagen type 1, bone sialoprotein and alpha-smooth muscle actin in the periodontal ligament. / Olson, C.; Uribe, F.; Kalajzic, Z.; Utreja, Achint; Nanda, R.; Rowe, D.; Wadhwa, S.

In: Orthodontics and Craniofacial Research, Vol. 15, No. 1, 02.2012, p. 52-61.

Research output: Contribution to journalArticle

Olson, C. ; Uribe, F. ; Kalajzic, Z. ; Utreja, Achint ; Nanda, R. ; Rowe, D. ; Wadhwa, S. / Orthodontic tooth movement causes decreased promoter expression of collagen type 1, bone sialoprotein and alpha-smooth muscle actin in the periodontal ligament. In: Orthodontics and Craniofacial Research. 2012 ; Vol. 15, No. 1. pp. 52-61.
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