Osteoblast differentiation during orthodontic tooth movement

Robert Holland, Carol Bain, Achint Utreja

Research output: Contribution to journalArticle

Abstract

Objectives: To analyse the expression of osteoblast differentiation markers and osteoclast activity in the periodontal ligament (PDL) following 2, 4 and 7 days of orthodontic tooth movement (OTM) in an animal model. Setting and Sample Population: Eighteen C57BL/6 wild-type mice. Material and Methods: For the OTM model, orthodontic force was applied to the maxillary right first molar using a closed-coil NiTi spring activated between the molar and incisors. The left side served as the control. Following OTM, the dissected tissues were scanned for micro-computed tomography (micro-CT) analysis and processed for histology. Histological stains included tartrate-resistant acid phosphatase (TRAP) staining for osteoclasts and immunohistochemistry for osteoblast markers alpha-smooth muscle actin (α-SMA), osteopontin (OP) and osteocalcin (OC). Results: Micro-CT analysis showed increasing OTM on days 2, 4 and 7 days as well as decrease in bone volume and per cent bone volume at 4 and 7 days. Statistically significant increases in osteoblast marker expression were seen in all groups when compared to the control. TRAP expression was highest at 4 and 7 days, α-SMA was highest at 2 days and OP/OC was highest at 4 days. Conclusion: During OTM, proliferation of pre-osteoblasts peaks at 2 days while mineralization of the osteoid peaks at 4 days. The osteoclast response is delayed.

Original languageEnglish (US)
JournalOrthodontics and Craniofacial Research
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

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Tooth Movement Techniques
Osteoblasts
Osteoclasts
Osteopontin
Osteocalcin
Bone and Bones
Periodontal Ligament
Differentiation Antigens
Incisor
Orthodontics
Smooth Muscle
Actins
Histology
Coloring Agents
Animal Models
Immunohistochemistry
Tomography
Staining and Labeling
Population

Keywords

  • orthodontic tooth movement
  • osteoblast
  • osteoclast

ASJC Scopus subject areas

  • Orthodontics
  • Surgery
  • Oral Surgery
  • Otorhinolaryngology

Cite this

Osteoblast differentiation during orthodontic tooth movement. / Holland, Robert; Bain, Carol; Utreja, Achint.

In: Orthodontics and Craniofacial Research, 01.01.2019.

Research output: Contribution to journalArticle

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