Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures

N. Takahashi, H. Yamana, S. Yoshiki, G. D. Roodman, G. David Roodman, S. J. Jones, A. Boyde, T. Suda

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Abstract

We developed a mouse bone marrow culture system to examine the process of osteoclast-like multinucleated cell formation from its progenitors. When mouse marrow cells were cultured for 8 days with 1α,25-dihydroxyvitamin D3 [1α,25-(OH)2D3, 10-10 to 10-7 M] or human PTH (1-34) (25-100 ng/ml), tartrate-resistant acid phosphatase (TRACP)-positive multinucleated cells formed. No TRACP-positive multinucleated cells appeared in the absence of these hormones. 1α,25-(OH)2D3 and PTH also increased the number of the clusters of TRACP-positive mononuclear cells. Time course studies showed that these TRACP-positive mononuclear cell clusters appeared before the formation of TRACP-positive multinucleated cells, suggesting that the TRACP-positive mononuclear cells are precursors of the multinucleated cells. Salmon calcitonin markedly inhibited the formation of TRACP-positive multinucleated cells but not TRACP-positive mononuclear cell clusters induced by 1α,25-(OH)2D3 or PTH. TRACP-positive mononuclear cells and multinucleated cells were rarely stained for nonspecific esterase, but some mononuclear cells were positively stained for both nonspecific esterase and TRACP. More that 90% of the TRACP-positive mononuclear cell clusters and multinucleated cells were found near colonies of alkaline phosphatase-positive mononuclear cells (possibly osteoblasts). When marrow mononuclear cells were cultured on sperm whale dentine slices in the presence of 1α,25-(OH)2D3 or PTH, numerous resorption lacunae were formed. These results suggest that 1) TRACP-positive multinucleated cells formed in response to osteotropic hormones in mouse marrow cultures satisfy most of the criteria of osteoclasts, and 2) osteoblasts may play an important role in osteoclast formation.

Original languageEnglish (US)
Pages (from-to)1373-1382
Number of pages10
JournalEndocrinology
Volume122
Issue number4
StatePublished - 1988
Externally publishedYes

Fingerprint

Osteoclasts
Bone Marrow
Hormones
Carboxylesterase
salmon calcitonin
Osteoblasts
Tartrate-Resistant Acid Phosphatase
Cultured Cells
Sperm Whale
Calcitriol
Dentin
Parathyroid Hormone

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Takahashi, N., Yamana, H., Yoshiki, S., Roodman, G. D., Roodman, G. D., Jones, S. J., ... Suda, T. (1988). Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. Endocrinology, 122(4), 1373-1382.

Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. / Takahashi, N.; Yamana, H.; Yoshiki, S.; Roodman, G. D.; Roodman, G. David; Jones, S. J.; Boyde, A.; Suda, T.

In: Endocrinology, Vol. 122, No. 4, 1988, p. 1373-1382.

Research output: Contribution to journalArticle

Takahashi, N, Yamana, H, Yoshiki, S, Roodman, GD, Roodman, GD, Jones, SJ, Boyde, A & Suda, T 1988, 'Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures', Endocrinology, vol. 122, no. 4, pp. 1373-1382.
Takahashi N, Yamana H, Yoshiki S, Roodman GD, Roodman GD, Jones SJ et al. Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. Endocrinology. 1988;122(4):1373-1382.
Takahashi, N. ; Yamana, H. ; Yoshiki, S. ; Roodman, G. D. ; Roodman, G. David ; Jones, S. J. ; Boyde, A. ; Suda, T. / Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. In: Endocrinology. 1988 ; Vol. 122, No. 4. pp. 1373-1382.
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