Osteocytic perilacunar/canalicular turnover in hemodialysis patients with high and low serum PTH levels

Aiji Yajima, Ken Tsuchiya, David Burr, Daniel E. Minner, Keith W. Condon, Caroline A. Miller, Shigeru Satoh, Masaaki Inaba, Takashi Nakayama, Tatsuhiko Tanizawa, Akemi Ito, Kosaku Nitta

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Osteocytic perilacunar/canalicular turnover in hemodialysis patients has not yet been reported. Osteocyte lacunae in lamellar bone and woven bone were classified as eroded surface-, osteoid surface-, and quiescent surface-predominant osteocyte lacunae (ES-Lc, OS-Lc, QS-Lc, respectively) in 55 hemodialysis patients with either high- (n = 45) or low- (n = 10) parathyroid hormone levels, and 19 control subjects without chronic kidney disease. We calculated the area and number of ES-Lc, OS-Lc, and QS-Lc. The mineralized surface on the osteocyte lacunar walls was measured in each group, and compared among the three groups. The shapes of the osteocyte lacunar walls were validated by backscattered electron microscopy. While the number of ES-Lc per bone area (N.ES-Lc/B.Ar) was higher than the number of OS-Lc per bone area (N.OS-Lc/B.Ar) in all groups, N.ES-Lc/B.Ar and N.OS-Lc/B.Ar were greater in high-parathyroid hormone group than in low-parathyroid hormone and control groups. The total volume of ES-Lc per bone area (ES-Lc.Ar/B.Ar) was greater than the total volume of OS-Lc per bone area (OS-Lc.Ar/B.Ar) in both parathyroid hormone groups. However, both lacunar erosion and lacunar formation increased proportionally, suggesting that global coupling between them was maintained. N.ES-Lc/B.Ar was higher in woven bone than in lamellar bone. The rate of OS-Lc stained by tetracycline hydrochloride, the mineralized lacunar surface and the mean area of OS-Lc with Tc obtained from both parathyroid hormone groups were greater than those in the control group. We conclude that osteocytic perilacunar/canalicular turnover is increased in hemodialysis patients with high parathyroid hormone levels. Osteocytic perilacunar/canalicular turnover depends, at least in part, on serum parathyroid hormone level. However, the ideal PTH level for osteocytic perilacunar/canalicular turnover could not be determined but osteocytic osteolysis was predominant in both the high- and low-PTH groups in this study. Thus, attention should be paid to bone loss from the viewpoint of osteocytic perilacunar/canalicular turnover in hemodialysis patients.

Original languageEnglish (US)
Pages (from-to)68-76
Number of pages9
JournalBone
Volume113
DOIs
StatePublished - Aug 1 2018

Fingerprint

Parathyroid Hormone
Renal Dialysis
Bone and Bones
Osteocytes
Serum
Control Groups
Osteolysis
Tetracycline
Chronic Renal Insufficiency
Electron Microscopy

Keywords

  • Backscattered electron microscope
  • Chronic kidney disease
  • Osteocyte lacunae
  • Osteocytic perilacunar/canalicular turnover
  • Parathyroid hormone

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Cite this

Yajima, A., Tsuchiya, K., Burr, D., Minner, D. E., Condon, K. W., Miller, C. A., ... Nitta, K. (2018). Osteocytic perilacunar/canalicular turnover in hemodialysis patients with high and low serum PTH levels. Bone, 113, 68-76. https://doi.org/10.1016/j.bone.2018.05.002

Osteocytic perilacunar/canalicular turnover in hemodialysis patients with high and low serum PTH levels. / Yajima, Aiji; Tsuchiya, Ken; Burr, David; Minner, Daniel E.; Condon, Keith W.; Miller, Caroline A.; Satoh, Shigeru; Inaba, Masaaki; Nakayama, Takashi; Tanizawa, Tatsuhiko; Ito, Akemi; Nitta, Kosaku.

In: Bone, Vol. 113, 01.08.2018, p. 68-76.

Research output: Contribution to journalArticle

Yajima, A, Tsuchiya, K, Burr, D, Minner, DE, Condon, KW, Miller, CA, Satoh, S, Inaba, M, Nakayama, T, Tanizawa, T, Ito, A & Nitta, K 2018, 'Osteocytic perilacunar/canalicular turnover in hemodialysis patients with high and low serum PTH levels', Bone, vol. 113, pp. 68-76. https://doi.org/10.1016/j.bone.2018.05.002
Yajima, Aiji ; Tsuchiya, Ken ; Burr, David ; Minner, Daniel E. ; Condon, Keith W. ; Miller, Caroline A. ; Satoh, Shigeru ; Inaba, Masaaki ; Nakayama, Takashi ; Tanizawa, Tatsuhiko ; Ito, Akemi ; Nitta, Kosaku. / Osteocytic perilacunar/canalicular turnover in hemodialysis patients with high and low serum PTH levels. In: Bone. 2018 ; Vol. 113. pp. 68-76.
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AU - Yajima, Aiji

AU - Tsuchiya, Ken

AU - Burr, David

AU - Minner, Daniel E.

AU - Condon, Keith W.

AU - Miller, Caroline A.

AU - Satoh, Shigeru

AU - Inaba, Masaaki

AU - Nakayama, Takashi

AU - Tanizawa, Tatsuhiko

AU - Ito, Akemi

AU - Nitta, Kosaku

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N2 - Osteocytic perilacunar/canalicular turnover in hemodialysis patients has not yet been reported. Osteocyte lacunae in lamellar bone and woven bone were classified as eroded surface-, osteoid surface-, and quiescent surface-predominant osteocyte lacunae (ES-Lc, OS-Lc, QS-Lc, respectively) in 55 hemodialysis patients with either high- (n = 45) or low- (n = 10) parathyroid hormone levels, and 19 control subjects without chronic kidney disease. We calculated the area and number of ES-Lc, OS-Lc, and QS-Lc. The mineralized surface on the osteocyte lacunar walls was measured in each group, and compared among the three groups. The shapes of the osteocyte lacunar walls were validated by backscattered electron microscopy. While the number of ES-Lc per bone area (N.ES-Lc/B.Ar) was higher than the number of OS-Lc per bone area (N.OS-Lc/B.Ar) in all groups, N.ES-Lc/B.Ar and N.OS-Lc/B.Ar were greater in high-parathyroid hormone group than in low-parathyroid hormone and control groups. The total volume of ES-Lc per bone area (ES-Lc.Ar/B.Ar) was greater than the total volume of OS-Lc per bone area (OS-Lc.Ar/B.Ar) in both parathyroid hormone groups. However, both lacunar erosion and lacunar formation increased proportionally, suggesting that global coupling between them was maintained. N.ES-Lc/B.Ar was higher in woven bone than in lamellar bone. The rate of OS-Lc stained by tetracycline hydrochloride, the mineralized lacunar surface and the mean area of OS-Lc with Tc obtained from both parathyroid hormone groups were greater than those in the control group. We conclude that osteocytic perilacunar/canalicular turnover is increased in hemodialysis patients with high parathyroid hormone levels. Osteocytic perilacunar/canalicular turnover depends, at least in part, on serum parathyroid hormone level. However, the ideal PTH level for osteocytic perilacunar/canalicular turnover could not be determined but osteocytic osteolysis was predominant in both the high- and low-PTH groups in this study. Thus, attention should be paid to bone loss from the viewpoint of osteocytic perilacunar/canalicular turnover in hemodialysis patients.

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KW - Backscattered electron microscope

KW - Chronic kidney disease

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KW - Osteocytic perilacunar/canalicular turnover

KW - Parathyroid hormone

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