Osteogenic differentiation of human umbilical cord mesenchymal stromal cells in polyglycolic acid scaffolds

Limin Wang, Nathan H. Dormer, Lynda Bonewald, Michael S. Detamore

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Although human umbilical cord mesenchymal stromal cells (hUCMSCs) have been shown to differentiate along an osteogenic lineage in monolayer culture, the potential of these cells has seldom before been investigated in three-dimensional scaffolds for bone tissue engineering applications. In this 6-week study, we observed osteogenic differentiation of hUCMSCs on polyglycolic acid (PGA) nonwoven mesh scaffolds, and compared seeding densities for potential use in bone tissue engineering. Cells were seeded into PGA meshes with densities of 5, 25, or 50 × 106 cells/mL scaffold and then cultured in osteogenic medium. Cell proliferation, osteogenic differentiation, and matrix formation were evaluated at weeks 0, 3, and 6. Osteogenic differentiation was observed based on positive alkaline phosphatase activity and an increase of collagen production and calcium incorporation into the extracellular matrix, which increased with higher cell density. During differentiation, runt-related transcription factor (RUNX2), type I collagen (CI), and osteocalcin (OCN) gene expression levels were also increased. In conclusion, exposed to osteogenic signals, hUCMSCs differentiated along an osteogenic lineage as determined by expression of osteogenic markers and matrix formation, and increasing the density of hUCMSCs seeded onto three-dimensional PGA scaffolds led to better osteogenic differentiation.

Original languageEnglish (US)
Pages (from-to)1937-1948
Number of pages12
JournalTissue Engineering - Part A
Volume16
Issue number6
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

Polyglycolic Acid
Umbilical Cord
Mesenchymal Stromal Cells
Scaffolds
Scaffolds (biology)
Acids
Tissue Engineering
Tissue engineering
Collagen
Bone
Bone and Bones
Transcription factors
Osteocalcin
Bioelectric potentials
Phosphatases
Cell proliferation
Collagen Type I
Gene expression
Extracellular Matrix
Alkaline Phosphatase

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Osteogenic differentiation of human umbilical cord mesenchymal stromal cells in polyglycolic acid scaffolds. / Wang, Limin; Dormer, Nathan H.; Bonewald, Lynda; Detamore, Michael S.

In: Tissue Engineering - Part A, Vol. 16, No. 6, 2010, p. 1937-1948.

Research output: Contribution to journalArticle

Wang, Limin ; Dormer, Nathan H. ; Bonewald, Lynda ; Detamore, Michael S. / Osteogenic differentiation of human umbilical cord mesenchymal stromal cells in polyglycolic acid scaffolds. In: Tissue Engineering - Part A. 2010 ; Vol. 16, No. 6. pp. 1937-1948.
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