Osteomacs interact with megakaryocytes and osteoblasts to regulate murine hematopoietic stem cell function

Safa F. Mohamad, Linlin Xu, Joydeep Ghosh, Paul J. Childress, Irushi Abeysekera, Evan R. Himes, Hao Wu, Marta B. Alvarez, Korbin M. Davis, Alexandra Aguilar-Perez, Jung Min Hong, Angela Bruzzaniti, Melissa A. Kacena, Edward F. Srour

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Networking between hematopoietic stem cells (HSCs) and cells of the hematopoietic niche is critical for stem cell function and maintenance of the stem cell pool. We characterized calvariae-resident osteomacs (OMs) and their interaction with megakaryocytes to sustain HSC function and identified distinguishing properties between OMs and bone marrow (BM)-derived macrophages. OMs, identified as CD451F4/801 cells, were easily detectable (3%-5%) in neonatal calvarial cells. Coculture of neonatal calvarial cells with megakaryocytes for 7 days increased OM three- to sixfold, demonstrating that megakaryocytes regulate OM proliferation. OMs were required for the hematopoiesis-enhancing activity of osteoblasts, and this activity was augmented by megakaryocytes. Serial transplantation demonstrated that HSC repopulating potential was best maintained by in vitro cultures containing osteoblasts, OMs, and megakaryocytes. With or without megakaryocytes, BM-derived macrophages were unable to functionally substitute for neonatal calvarial cell-associated OMs. In addition, OMs differentiated into multinucleated, tartrate resistant acid phosphatase-positive osteoclasts capable of bone resorption. Nine-color flow cytometric analysis revealed that although BM-derived macrophages and OMs share many cell surface phenotypic similarities (CD45, F4/80, CD68, CD11b, Mac2, and Gr-1), only a subgroup of OMs coexpressed M-CSFR and CD166, thus providing a unique profile for OMs. CD169 was expressed by both OMs and BM-derived macrophages and therefore was not a distinguishing marker between these 2 cell types. These results demonstrate that OMs support HSC function and illustrate that megakaryocytes significantly augment the synergistic activity of osteoblasts and OMs. Furthermore, this report establishes for the first time that the crosstalk between OMs, osteoblasts, and megakaryocytes is a novel network supporting HSC function.

Original languageEnglish (US)
Pages (from-to)2520-2528
Number of pages9
JournalBlood Advances
Volume1
Issue number26
DOIs
StatePublished - Dec 12 2017

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Megakaryocytes
Hematopoietic Stem Cells
Osteoblasts
Macrophages
Stem Cells
Hematopoietic Stem Cell Transplantation
Hematopoiesis
Osteoclasts
Bone Resorption
Coculture Techniques
Skull
Color
Maintenance

ASJC Scopus subject areas

  • Hematology

Cite this

Osteomacs interact with megakaryocytes and osteoblasts to regulate murine hematopoietic stem cell function. / Mohamad, Safa F.; Xu, Linlin; Ghosh, Joydeep; Childress, Paul J.; Abeysekera, Irushi; Himes, Evan R.; Wu, Hao; Alvarez, Marta B.; Davis, Korbin M.; Aguilar-Perez, Alexandra; Hong, Jung Min; Bruzzaniti, Angela; Kacena, Melissa A.; Srour, Edward F.

In: Blood Advances, Vol. 1, No. 26, 12.12.2017, p. 2520-2528.

Research output: Contribution to journalArticle

Mohamad, SF, Xu, L, Ghosh, J, Childress, PJ, Abeysekera, I, Himes, ER, Wu, H, Alvarez, MB, Davis, KM, Aguilar-Perez, A, Hong, JM, Bruzzaniti, A, Kacena, MA & Srour, EF 2017, 'Osteomacs interact with megakaryocytes and osteoblasts to regulate murine hematopoietic stem cell function', Blood Advances, vol. 1, no. 26, pp. 2520-2528. https://doi.org/10.1182/bloodadvances.2017011304
Mohamad, Safa F. ; Xu, Linlin ; Ghosh, Joydeep ; Childress, Paul J. ; Abeysekera, Irushi ; Himes, Evan R. ; Wu, Hao ; Alvarez, Marta B. ; Davis, Korbin M. ; Aguilar-Perez, Alexandra ; Hong, Jung Min ; Bruzzaniti, Angela ; Kacena, Melissa A. ; Srour, Edward F. / Osteomacs interact with megakaryocytes and osteoblasts to regulate murine hematopoietic stem cell function. In: Blood Advances. 2017 ; Vol. 1, No. 26. pp. 2520-2528.
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abstract = "Networking between hematopoietic stem cells (HSCs) and cells of the hematopoietic niche is critical for stem cell function and maintenance of the stem cell pool. We characterized calvariae-resident osteomacs (OMs) and their interaction with megakaryocytes to sustain HSC function and identified distinguishing properties between OMs and bone marrow (BM)-derived macrophages. OMs, identified as CD451F4/801 cells, were easily detectable (3{\%}-5{\%}) in neonatal calvarial cells. Coculture of neonatal calvarial cells with megakaryocytes for 7 days increased OM three- to sixfold, demonstrating that megakaryocytes regulate OM proliferation. OMs were required for the hematopoiesis-enhancing activity of osteoblasts, and this activity was augmented by megakaryocytes. Serial transplantation demonstrated that HSC repopulating potential was best maintained by in vitro cultures containing osteoblasts, OMs, and megakaryocytes. With or without megakaryocytes, BM-derived macrophages were unable to functionally substitute for neonatal calvarial cell-associated OMs. In addition, OMs differentiated into multinucleated, tartrate resistant acid phosphatase-positive osteoclasts capable of bone resorption. Nine-color flow cytometric analysis revealed that although BM-derived macrophages and OMs share many cell surface phenotypic similarities (CD45, F4/80, CD68, CD11b, Mac2, and Gr-1), only a subgroup of OMs coexpressed M-CSFR and CD166, thus providing a unique profile for OMs. CD169 was expressed by both OMs and BM-derived macrophages and therefore was not a distinguishing marker between these 2 cell types. These results demonstrate that OMs support HSC function and illustrate that megakaryocytes significantly augment the synergistic activity of osteoblasts and OMs. Furthermore, this report establishes for the first time that the crosstalk between OMs, osteoblasts, and megakaryocytes is a novel network supporting HSC function.",
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AU - Xu, Linlin

AU - Ghosh, Joydeep

AU - Childress, Paul J.

AU - Abeysekera, Irushi

AU - Himes, Evan R.

AU - Wu, Hao

AU - Alvarez, Marta B.

AU - Davis, Korbin M.

AU - Aguilar-Perez, Alexandra

AU - Hong, Jung Min

AU - Bruzzaniti, Angela

AU - Kacena, Melissa A.

AU - Srour, Edward F.

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