Ovarian hormone depletion affects cortical bone quality differently on different skeletal envelopes

E. P. Paschalis, S. Gamsjaeger, N. Hassler, K. Klaushofer, David Burr

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The physical properties of bone tissue are determined by the organic and mineral matrix, and are one aspect of bone quality. As such, the properties of mineral and matrix are a major contributor to bone strength, independent of bone mass. Cortical bone quality may differ regionally on the three skeletal envelopes that compose it. Each of these envelopes may be affected differently by ovarian hormone depletion. Identifying how these regions vary in their tissue adaptive response to ovarian hormones can inform our understanding of how tissue quality contributes to overall bone strength in postmenopausal women. We analyzed humeri from monkeys that were either SHAM-operated or ovariectomized. Raman microspectroscopic analysis was performed as a function of tissue age based on the presence of multiple fluorescent double labels, to determine whether bone compositional properties (mineral/matrix ratio, tissue water, glycosaminoglycan, lipid, and pyridinoline contents, and mineral maturity/crystallinity) are similar between periosteal, osteonal, and endosteal surfaces, as well as to determine the effects of ovarian hormone depletion on them. The results indicate that mineral and organic matrix characteristics, and kinetics of mineral and organic matrix modifications as a function of tissue age are different at periosteal vs. osteonal and endosteal surfaces. Ovarian hormone depletion affects the three cortical surfaces (periosteal, osteonal, endosteal) differently. While ovarian hormone depletion does not significantly affect the quality of either the osteoid or the most recently mineralized tissue, it significantly affects the rate of subsequent mineral accumulation, as well as the kinetics of organic matrix modifications, culminating in significant differences within interstitial bone. These results highlight the complexity of the cortical bone compartments, add to existing knowledge on the effects of ovarian hormone depletion on local cortical bone properties, and may contribute to a better understanding of the location specific action of drugs used in the management of postmenopausal osteoporosis.

Original languageEnglish (US)
Pages (from-to)55-64
Number of pages10
JournalBone
Volume95
DOIs
StatePublished - Feb 1 2017

Fingerprint

Minerals
Hormones
Bone and Bones
Postmenopausal Osteoporosis
Humerus
Cortical Bone
Glycosaminoglycans
Haplorhini
Lipids
Water
Pharmaceutical Preparations

Keywords

  • Bone quality
  • Cortical bone
  • Humerus
  • Osteoporosis
  • Ovariectomy
  • Raman spectroscopy

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Histology
  • Physiology

Cite this

Paschalis, E. P., Gamsjaeger, S., Hassler, N., Klaushofer, K., & Burr, D. (2017). Ovarian hormone depletion affects cortical bone quality differently on different skeletal envelopes. Bone, 95, 55-64. https://doi.org/10.1016/j.bone.2016.10.029

Ovarian hormone depletion affects cortical bone quality differently on different skeletal envelopes. / Paschalis, E. P.; Gamsjaeger, S.; Hassler, N.; Klaushofer, K.; Burr, David.

In: Bone, Vol. 95, 01.02.2017, p. 55-64.

Research output: Contribution to journalArticle

Paschalis, EP, Gamsjaeger, S, Hassler, N, Klaushofer, K & Burr, D 2017, 'Ovarian hormone depletion affects cortical bone quality differently on different skeletal envelopes', Bone, vol. 95, pp. 55-64. https://doi.org/10.1016/j.bone.2016.10.029
Paschalis, E. P. ; Gamsjaeger, S. ; Hassler, N. ; Klaushofer, K. ; Burr, David. / Ovarian hormone depletion affects cortical bone quality differently on different skeletal envelopes. In: Bone. 2017 ; Vol. 95. pp. 55-64.
@article{ab5b723a949b4e6b902a53833f5b7dfc,
title = "Ovarian hormone depletion affects cortical bone quality differently on different skeletal envelopes",
abstract = "The physical properties of bone tissue are determined by the organic and mineral matrix, and are one aspect of bone quality. As such, the properties of mineral and matrix are a major contributor to bone strength, independent of bone mass. Cortical bone quality may differ regionally on the three skeletal envelopes that compose it. Each of these envelopes may be affected differently by ovarian hormone depletion. Identifying how these regions vary in their tissue adaptive response to ovarian hormones can inform our understanding of how tissue quality contributes to overall bone strength in postmenopausal women. We analyzed humeri from monkeys that were either SHAM-operated or ovariectomized. Raman microspectroscopic analysis was performed as a function of tissue age based on the presence of multiple fluorescent double labels, to determine whether bone compositional properties (mineral/matrix ratio, tissue water, glycosaminoglycan, lipid, and pyridinoline contents, and mineral maturity/crystallinity) are similar between periosteal, osteonal, and endosteal surfaces, as well as to determine the effects of ovarian hormone depletion on them. The results indicate that mineral and organic matrix characteristics, and kinetics of mineral and organic matrix modifications as a function of tissue age are different at periosteal vs. osteonal and endosteal surfaces. Ovarian hormone depletion affects the three cortical surfaces (periosteal, osteonal, endosteal) differently. While ovarian hormone depletion does not significantly affect the quality of either the osteoid or the most recently mineralized tissue, it significantly affects the rate of subsequent mineral accumulation, as well as the kinetics of organic matrix modifications, culminating in significant differences within interstitial bone. These results highlight the complexity of the cortical bone compartments, add to existing knowledge on the effects of ovarian hormone depletion on local cortical bone properties, and may contribute to a better understanding of the location specific action of drugs used in the management of postmenopausal osteoporosis.",
keywords = "Bone quality, Cortical bone, Humerus, Osteoporosis, Ovariectomy, Raman spectroscopy",
author = "Paschalis, {E. P.} and S. Gamsjaeger and N. Hassler and K. Klaushofer and David Burr",
year = "2017",
month = "2",
day = "1",
doi = "10.1016/j.bone.2016.10.029",
language = "English (US)",
volume = "95",
pages = "55--64",
journal = "Bone",
issn = "8756-3282",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Ovarian hormone depletion affects cortical bone quality differently on different skeletal envelopes

AU - Paschalis, E. P.

AU - Gamsjaeger, S.

AU - Hassler, N.

AU - Klaushofer, K.

AU - Burr, David

PY - 2017/2/1

Y1 - 2017/2/1

N2 - The physical properties of bone tissue are determined by the organic and mineral matrix, and are one aspect of bone quality. As such, the properties of mineral and matrix are a major contributor to bone strength, independent of bone mass. Cortical bone quality may differ regionally on the three skeletal envelopes that compose it. Each of these envelopes may be affected differently by ovarian hormone depletion. Identifying how these regions vary in their tissue adaptive response to ovarian hormones can inform our understanding of how tissue quality contributes to overall bone strength in postmenopausal women. We analyzed humeri from monkeys that were either SHAM-operated or ovariectomized. Raman microspectroscopic analysis was performed as a function of tissue age based on the presence of multiple fluorescent double labels, to determine whether bone compositional properties (mineral/matrix ratio, tissue water, glycosaminoglycan, lipid, and pyridinoline contents, and mineral maturity/crystallinity) are similar between periosteal, osteonal, and endosteal surfaces, as well as to determine the effects of ovarian hormone depletion on them. The results indicate that mineral and organic matrix characteristics, and kinetics of mineral and organic matrix modifications as a function of tissue age are different at periosteal vs. osteonal and endosteal surfaces. Ovarian hormone depletion affects the three cortical surfaces (periosteal, osteonal, endosteal) differently. While ovarian hormone depletion does not significantly affect the quality of either the osteoid or the most recently mineralized tissue, it significantly affects the rate of subsequent mineral accumulation, as well as the kinetics of organic matrix modifications, culminating in significant differences within interstitial bone. These results highlight the complexity of the cortical bone compartments, add to existing knowledge on the effects of ovarian hormone depletion on local cortical bone properties, and may contribute to a better understanding of the location specific action of drugs used in the management of postmenopausal osteoporosis.

AB - The physical properties of bone tissue are determined by the organic and mineral matrix, and are one aspect of bone quality. As such, the properties of mineral and matrix are a major contributor to bone strength, independent of bone mass. Cortical bone quality may differ regionally on the three skeletal envelopes that compose it. Each of these envelopes may be affected differently by ovarian hormone depletion. Identifying how these regions vary in their tissue adaptive response to ovarian hormones can inform our understanding of how tissue quality contributes to overall bone strength in postmenopausal women. We analyzed humeri from monkeys that were either SHAM-operated or ovariectomized. Raman microspectroscopic analysis was performed as a function of tissue age based on the presence of multiple fluorescent double labels, to determine whether bone compositional properties (mineral/matrix ratio, tissue water, glycosaminoglycan, lipid, and pyridinoline contents, and mineral maturity/crystallinity) are similar between periosteal, osteonal, and endosteal surfaces, as well as to determine the effects of ovarian hormone depletion on them. The results indicate that mineral and organic matrix characteristics, and kinetics of mineral and organic matrix modifications as a function of tissue age are different at periosteal vs. osteonal and endosteal surfaces. Ovarian hormone depletion affects the three cortical surfaces (periosteal, osteonal, endosteal) differently. While ovarian hormone depletion does not significantly affect the quality of either the osteoid or the most recently mineralized tissue, it significantly affects the rate of subsequent mineral accumulation, as well as the kinetics of organic matrix modifications, culminating in significant differences within interstitial bone. These results highlight the complexity of the cortical bone compartments, add to existing knowledge on the effects of ovarian hormone depletion on local cortical bone properties, and may contribute to a better understanding of the location specific action of drugs used in the management of postmenopausal osteoporosis.

KW - Bone quality

KW - Cortical bone

KW - Humerus

KW - Osteoporosis

KW - Ovariectomy

KW - Raman spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=84995553839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84995553839&partnerID=8YFLogxK

U2 - 10.1016/j.bone.2016.10.029

DO - 10.1016/j.bone.2016.10.029

M3 - Article

VL - 95

SP - 55

EP - 64

JO - Bone

JF - Bone

SN - 8756-3282

ER -