Overexpression of glycogen synthase in mouse muscle results in less branched glycogen

Bartholomew A. Pederson, Anna G. Csitkovits, Renee Simon, Jill M. Schroeder, Wei Wang, Alexander Skurat, Peter Roach

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Glycogen, a branched polymer of glucose, serves as an energy reserve in many organisms. The degree of branching likely reflects the balance between the activities of glycogen synthase and branching enzyme. Mice overexpressing constitutively active glycogen synthase in skeletal muscle (GSL30) have elevated muscle glycogen. To test whether excess glycogen synthase activity affected glycogen branching, we examined the glycogen from skeletal muscle of GSL30 mice. The absorption spectrum of muscle glycogen determined in the presence of iodine was shifted to higher wavelengths in the GSL30 animals, consistent with a decrease in the degree of branching. As judged by Western blotting, the levels of glycogenin and the branching enzyme were also elevated. Branching enzyme activity also increased approximately threefold. However, this compared with an increase in glycogen synthase of some 50-fold, so that the increase in branching enzyme in response to overexpression of glycogen synthase was insufficient to synthesize normally branched glycogen.

Original languageEnglish
Pages (from-to)826-830
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume305
Issue number4
DOIs
StatePublished - Jun 13 2003

Fingerprint

Glycogen Synthase
1,4-alpha-Glucan Branching Enzyme
Glycogen
Muscle
Muscles
Skeletal Muscle
Glucans
Enzyme activity
Iodine
Absorption spectra
Animals
Western Blotting
Wavelength

Keywords

  • Branching enzyme
  • Glycogen
  • Glycogen synthase
  • Glycogenin
  • Lafora

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Overexpression of glycogen synthase in mouse muscle results in less branched glycogen. / Pederson, Bartholomew A.; Csitkovits, Anna G.; Simon, Renee; Schroeder, Jill M.; Wang, Wei; Skurat, Alexander; Roach, Peter.

In: Biochemical and Biophysical Research Communications, Vol. 305, No. 4, 13.06.2003, p. 826-830.

Research output: Contribution to journalArticle

Pederson, Bartholomew A. ; Csitkovits, Anna G. ; Simon, Renee ; Schroeder, Jill M. ; Wang, Wei ; Skurat, Alexander ; Roach, Peter. / Overexpression of glycogen synthase in mouse muscle results in less branched glycogen. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 305, No. 4. pp. 826-830.
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