Overexpression of HER2/neu oncogene in human pancreatic carcinoma

Yoichiro Yamanaka, Helmut Friess, Michael S. Kobrin, Markus Büchler, Jürgen Kunz, Hans G. Beger, Murray Korc

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

The HER2/neu oncogene encodes a transmembrane protein that possesses intrinsic tyrosine kinase activity. Its overexpression has been associated with the malignant phenotype. In this study we examined HER2/neu expression in the normal and cancerous human pancreas. In the normal pancreas HER2/neu immunostaining was observed in acinar and ductal cells. HER2/neu immunoreactivity was expressed in 34 of 76 (45%) pancreatic carcinomas. There was a significant correlation between tumors with well-differentiated histology and HER2/neu expression. Northern blot analysis demonstrated HER2/neu mRNA expression in the normal pancreas and in situ hybridization confirmed its distribution in both acinar and ductal cells. In cancer tissues Northern blot analysis indicated that HER2/neu mRNA levels were elevated in 13 of 25 (52%) of the tumors in comparison with the normal tissues. In addition, in situ hybridization demonstrated a strong but heterogenous distribution of mRNA grains in these tumors. Southern blot analysis did not demonstrate HER2/neu gene amplification in any of the tumors. These data indicate that the HER2/neu protein is synthesized in the normal exocrine pancreas and is frequently overexpressed in well-differentiated adenocarcinomas of the pancreas as a result of increased HER2/neu mRNA levels.

Original languageEnglish (US)
Pages (from-to)1127-1134
Number of pages8
JournalHuman Pathology
Volume24
Issue number10
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Oncogenes
Pancreas
erbB-2 Genes
Messenger RNA
Acinar Cells
Neoplasms
Northern Blotting
In Situ Hybridization
Exocrine Pancreas
Gene Amplification
Southern Blotting
Protein-Tyrosine Kinases
Histology
Proteins
Adenocarcinoma
Pancreatic Carcinoma
Phenotype

Keywords

  • clinicopathologic parameters
  • immunohistochemistry
  • in situ hybridization
  • Northern blot analysis
  • Southern blot analysis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Yamanaka, Y., Friess, H., Kobrin, M. S., Büchler, M., Kunz, J., Beger, H. G., & Korc, M. (1993). Overexpression of HER2/neu oncogene in human pancreatic carcinoma. Human Pathology, 24(10), 1127-1134. https://doi.org/10.1016/0046-8177(93)90194-L

Overexpression of HER2/neu oncogene in human pancreatic carcinoma. / Yamanaka, Yoichiro; Friess, Helmut; Kobrin, Michael S.; Büchler, Markus; Kunz, Jürgen; Beger, Hans G.; Korc, Murray.

In: Human Pathology, Vol. 24, No. 10, 1993, p. 1127-1134.

Research output: Contribution to journalArticle

Yamanaka, Y, Friess, H, Kobrin, MS, Büchler, M, Kunz, J, Beger, HG & Korc, M 1993, 'Overexpression of HER2/neu oncogene in human pancreatic carcinoma', Human Pathology, vol. 24, no. 10, pp. 1127-1134. https://doi.org/10.1016/0046-8177(93)90194-L
Yamanaka Y, Friess H, Kobrin MS, Büchler M, Kunz J, Beger HG et al. Overexpression of HER2/neu oncogene in human pancreatic carcinoma. Human Pathology. 1993;24(10):1127-1134. https://doi.org/10.1016/0046-8177(93)90194-L
Yamanaka, Yoichiro ; Friess, Helmut ; Kobrin, Michael S. ; Büchler, Markus ; Kunz, Jürgen ; Beger, Hans G. ; Korc, Murray. / Overexpression of HER2/neu oncogene in human pancreatic carcinoma. In: Human Pathology. 1993 ; Vol. 24, No. 10. pp. 1127-1134.
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