Overexpression of HER2/neu oncogene in human pancreatic carcinoma

Yoichiro Yamanaka, Helmut Friess, Michael S. Kobrin, Markus Büchler, Jürgen Kunz, Hans G. Beger, Murray Korc

Research output: Contribution to journalArticle

198 Scopus citations

Abstract

The HER2/neu oncogene encodes a transmembrane protein that possesses intrinsic tyrosine kinase activity. Its overexpression has been associated with the malignant phenotype. In this study we examined HER2/neu expression in the normal and cancerous human pancreas. In the normal pancreas HER2/neu immunostaining was observed in acinar and ductal cells. HER2/neu immunoreactivity was expressed in 34 of 76 (45%) pancreatic carcinomas. There was a significant correlation between tumors with well-differentiated histology and HER2/neu expression. Northern blot analysis demonstrated HER2/neu mRNA expression in the normal pancreas and in situ hybridization confirmed its distribution in both acinar and ductal cells. In cancer tissues Northern blot analysis indicated that HER2/neu mRNA levels were elevated in 13 of 25 (52%) of the tumors in comparison with the normal tissues. In addition, in situ hybridization demonstrated a strong but heterogenous distribution of mRNA grains in these tumors. Southern blot analysis did not demonstrate HER2/neu gene amplification in any of the tumors. These data indicate that the HER2/neu protein is synthesized in the normal exocrine pancreas and is frequently overexpressed in well-differentiated adenocarcinomas of the pancreas as a result of increased HER2/neu mRNA levels.

Original languageEnglish (US)
Pages (from-to)1127-1134
Number of pages8
JournalHuman pathology
Volume24
Issue number10
DOIs
StatePublished - Oct 1993

Keywords

  • Northern blot analysis
  • Southern blot analysis
  • clinicopathologic parameters
  • immunohistochemistry
  • in situ hybridization

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Yamanaka, Y., Friess, H., Kobrin, M. S., Büchler, M., Kunz, J., Beger, H. G., & Korc, M. (1993). Overexpression of HER2/neu oncogene in human pancreatic carcinoma. Human pathology, 24(10), 1127-1134. https://doi.org/10.1016/0046-8177(93)90194-L