Overexpression of lymphangiogenic growth factor VEGF-C in human pancreatic cancer

Rui Feng Tang, Jun Itakura, Takuma Aikawa, Kei Matsuda, Hideki Fujii, Murray Korc, Yoshiro Matsumoto

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Vascular endothelial growth factor C (VEGF-C) is a lymphangiogenic polypeptide that has been implicated in cancer growth. In this study, we characterized VEGF-C expression in cultured human pancreatic cancer cell lines and determined whether the presence of VEGF-C in human pancreatic cancers is associated with clinicopathologic characteristics. VEGF-C mRNA transcripts were present in all five tested cell lines (Capan-1, MIA-PaCa-2, PANC-1, COLO-357, and T3M4). Immunoblotting with a highly specific anti-VEGF-C antibody revealed the presence of VEGF-C protein in all the cell lines. Northern blot analysis of total RNA revealed an approximately 2.2-fold increase in VEGF-C mRNA transcript in the cancer samples compared with the normal pancreas. Immunohistochemical analysis confirmed the expression of VEGF-C and its receptor flt-4 in the cancer cells within the tumor mass. Immunohistochemical analysis of 51 pancreatic cancer tissues revealed the presence of strong VEGF-C immunoreactivity in the cancer cells in 80.4% of the cancer tissues. The presence of VEGF-C in these cells was associated with increased lymphatic vessels invasion and lymph node metastasis, but not with decreased patient survival. These findings indicate that VEGF-C and its receptor are commonly overexpressed in human pancreatic cancers and that this factor may contribute to the lymphangiogenic process and metastasis in this disorder.

Original languageEnglish (US)
Pages (from-to)285-292
Number of pages8
JournalPancreas
Volume22
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Vascular Endothelial Growth Factor C
Pancreatic Neoplasms
Intercellular Signaling Peptides and Proteins
Neoplasms
Cell Line
Vascular Endothelial Growth Factor Receptor-3
human VEGFC protein
Neoplasm Metastasis
Messenger RNA
Lymphatic Vessels
Immunoblotting
Northern Blotting
Pancreas
Lymph Nodes
RNA

Keywords

  • Flt-4
  • Human pancreatic cancer
  • Lymphangiogenesis
  • Vascular endothelial growth factor C

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology

Cite this

Tang, R. F., Itakura, J., Aikawa, T., Matsuda, K., Fujii, H., Korc, M., & Matsumoto, Y. (2001). Overexpression of lymphangiogenic growth factor VEGF-C in human pancreatic cancer. Pancreas, 22(3), 285-292. https://doi.org/10.1097/00006676-200104000-00010

Overexpression of lymphangiogenic growth factor VEGF-C in human pancreatic cancer. / Tang, Rui Feng; Itakura, Jun; Aikawa, Takuma; Matsuda, Kei; Fujii, Hideki; Korc, Murray; Matsumoto, Yoshiro.

In: Pancreas, Vol. 22, No. 3, 2001, p. 285-292.

Research output: Contribution to journalArticle

Tang, RF, Itakura, J, Aikawa, T, Matsuda, K, Fujii, H, Korc, M & Matsumoto, Y 2001, 'Overexpression of lymphangiogenic growth factor VEGF-C in human pancreatic cancer', Pancreas, vol. 22, no. 3, pp. 285-292. https://doi.org/10.1097/00006676-200104000-00010
Tang, Rui Feng ; Itakura, Jun ; Aikawa, Takuma ; Matsuda, Kei ; Fujii, Hideki ; Korc, Murray ; Matsumoto, Yoshiro. / Overexpression of lymphangiogenic growth factor VEGF-C in human pancreatic cancer. In: Pancreas. 2001 ; Vol. 22, No. 3. pp. 285-292.
@article{d926628b4c2242309e1a63cfe2e928d4,
title = "Overexpression of lymphangiogenic growth factor VEGF-C in human pancreatic cancer",
abstract = "Vascular endothelial growth factor C (VEGF-C) is a lymphangiogenic polypeptide that has been implicated in cancer growth. In this study, we characterized VEGF-C expression in cultured human pancreatic cancer cell lines and determined whether the presence of VEGF-C in human pancreatic cancers is associated with clinicopathologic characteristics. VEGF-C mRNA transcripts were present in all five tested cell lines (Capan-1, MIA-PaCa-2, PANC-1, COLO-357, and T3M4). Immunoblotting with a highly specific anti-VEGF-C antibody revealed the presence of VEGF-C protein in all the cell lines. Northern blot analysis of total RNA revealed an approximately 2.2-fold increase in VEGF-C mRNA transcript in the cancer samples compared with the normal pancreas. Immunohistochemical analysis confirmed the expression of VEGF-C and its receptor flt-4 in the cancer cells within the tumor mass. Immunohistochemical analysis of 51 pancreatic cancer tissues revealed the presence of strong VEGF-C immunoreactivity in the cancer cells in 80.4{\%} of the cancer tissues. The presence of VEGF-C in these cells was associated with increased lymphatic vessels invasion and lymph node metastasis, but not with decreased patient survival. These findings indicate that VEGF-C and its receptor are commonly overexpressed in human pancreatic cancers and that this factor may contribute to the lymphangiogenic process and metastasis in this disorder.",
keywords = "Flt-4, Human pancreatic cancer, Lymphangiogenesis, Vascular endothelial growth factor C",
author = "Tang, {Rui Feng} and Jun Itakura and Takuma Aikawa and Kei Matsuda and Hideki Fujii and Murray Korc and Yoshiro Matsumoto",
year = "2001",
doi = "10.1097/00006676-200104000-00010",
language = "English (US)",
volume = "22",
pages = "285--292",
journal = "Pancreas",
issn = "0885-3177",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Overexpression of lymphangiogenic growth factor VEGF-C in human pancreatic cancer

AU - Tang, Rui Feng

AU - Itakura, Jun

AU - Aikawa, Takuma

AU - Matsuda, Kei

AU - Fujii, Hideki

AU - Korc, Murray

AU - Matsumoto, Yoshiro

PY - 2001

Y1 - 2001

N2 - Vascular endothelial growth factor C (VEGF-C) is a lymphangiogenic polypeptide that has been implicated in cancer growth. In this study, we characterized VEGF-C expression in cultured human pancreatic cancer cell lines and determined whether the presence of VEGF-C in human pancreatic cancers is associated with clinicopathologic characteristics. VEGF-C mRNA transcripts were present in all five tested cell lines (Capan-1, MIA-PaCa-2, PANC-1, COLO-357, and T3M4). Immunoblotting with a highly specific anti-VEGF-C antibody revealed the presence of VEGF-C protein in all the cell lines. Northern blot analysis of total RNA revealed an approximately 2.2-fold increase in VEGF-C mRNA transcript in the cancer samples compared with the normal pancreas. Immunohistochemical analysis confirmed the expression of VEGF-C and its receptor flt-4 in the cancer cells within the tumor mass. Immunohistochemical analysis of 51 pancreatic cancer tissues revealed the presence of strong VEGF-C immunoreactivity in the cancer cells in 80.4% of the cancer tissues. The presence of VEGF-C in these cells was associated with increased lymphatic vessels invasion and lymph node metastasis, but not with decreased patient survival. These findings indicate that VEGF-C and its receptor are commonly overexpressed in human pancreatic cancers and that this factor may contribute to the lymphangiogenic process and metastasis in this disorder.

AB - Vascular endothelial growth factor C (VEGF-C) is a lymphangiogenic polypeptide that has been implicated in cancer growth. In this study, we characterized VEGF-C expression in cultured human pancreatic cancer cell lines and determined whether the presence of VEGF-C in human pancreatic cancers is associated with clinicopathologic characteristics. VEGF-C mRNA transcripts were present in all five tested cell lines (Capan-1, MIA-PaCa-2, PANC-1, COLO-357, and T3M4). Immunoblotting with a highly specific anti-VEGF-C antibody revealed the presence of VEGF-C protein in all the cell lines. Northern blot analysis of total RNA revealed an approximately 2.2-fold increase in VEGF-C mRNA transcript in the cancer samples compared with the normal pancreas. Immunohistochemical analysis confirmed the expression of VEGF-C and its receptor flt-4 in the cancer cells within the tumor mass. Immunohistochemical analysis of 51 pancreatic cancer tissues revealed the presence of strong VEGF-C immunoreactivity in the cancer cells in 80.4% of the cancer tissues. The presence of VEGF-C in these cells was associated with increased lymphatic vessels invasion and lymph node metastasis, but not with decreased patient survival. These findings indicate that VEGF-C and its receptor are commonly overexpressed in human pancreatic cancers and that this factor may contribute to the lymphangiogenic process and metastasis in this disorder.

KW - Flt-4

KW - Human pancreatic cancer

KW - Lymphangiogenesis

KW - Vascular endothelial growth factor C

UR - http://www.scopus.com/inward/record.url?scp=0035086990&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035086990&partnerID=8YFLogxK

U2 - 10.1097/00006676-200104000-00010

DO - 10.1097/00006676-200104000-00010

M3 - Article

C2 - 11291931

AN - SCOPUS:0035086990

VL - 22

SP - 285

EP - 292

JO - Pancreas

JF - Pancreas

SN - 0885-3177

IS - 3

ER -