Abstract
The epidermal growth factor (EGF) receptor is activated by both EGF and transforming growth factor-alpha (TGF-α). Using immunohistochemical and immunoblotting techniques we now report that the EGF receptor, EGF, and TGF-α are found in both pancreatic acini and ducts in the normal human pancreas, and that all three proteins are expressed at higher levels in human pancreatic cancer tissues. Using in situ hybridization techniques, we also report that the mRNA encoding the EGF receptor, EGF, and TGF-α colocalize with their respective proteins. Northern blot analysis of total RNA indicates that, by comparison with the normal pancreas, the pancreatic tumors exhibit a 3-, 15-, and 10-fold increase in the mRNA levels encoding the EGF receptor, EGF, and TGF-α, respectively. Furthermore, by in situ hybridization, there is a marked increase in these mRNA moieties within the tumor mass. These findings suggest that EGF and TGF-α may participate in the regulation of normal pancreatic exocrine function, and that overexpression of the EGF receptor and its two principal ligands may contribute to the pathophysiological processes that occur in human pancreatic cancer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1352-1360 |
| Number of pages | 9 |
| Journal | Journal of Clinical Investigation |
| Volume | 90 |
| Issue number | 4 |
| State | Published - Oct 1992 |
| Externally published | Yes |
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Keywords
- Autocrine
- Immunofluorescence
- In situ hybridization
- Northern blots
- Pancreas
ASJC Scopus subject areas
- Medicine(all)
Cite this
Overexpression of the epidermal growth factor receptor in human pancreatic cancer is associated with concomitant increases in the levels of epidermal growth factor and transforming growth factor alpha. / Korc, Murray; Chandrasekar, Bysani; Yamanaka, Yoichiro; Friess, Helmut; Buchler, Markus; Beger, Hans G.
In: Journal of Clinical Investigation, Vol. 90, No. 4, 10.1992, p. 1352-1360.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Overexpression of the epidermal growth factor receptor in human pancreatic cancer is associated with concomitant increases in the levels of epidermal growth factor and transforming growth factor alpha
AU - Korc, Murray
AU - Chandrasekar, Bysani
AU - Yamanaka, Yoichiro
AU - Friess, Helmut
AU - Buchler, Markus
AU - Beger, Hans G.
PY - 1992/10
Y1 - 1992/10
N2 - The epidermal growth factor (EGF) receptor is activated by both EGF and transforming growth factor-alpha (TGF-α). Using immunohistochemical and immunoblotting techniques we now report that the EGF receptor, EGF, and TGF-α are found in both pancreatic acini and ducts in the normal human pancreas, and that all three proteins are expressed at higher levels in human pancreatic cancer tissues. Using in situ hybridization techniques, we also report that the mRNA encoding the EGF receptor, EGF, and TGF-α colocalize with their respective proteins. Northern blot analysis of total RNA indicates that, by comparison with the normal pancreas, the pancreatic tumors exhibit a 3-, 15-, and 10-fold increase in the mRNA levels encoding the EGF receptor, EGF, and TGF-α, respectively. Furthermore, by in situ hybridization, there is a marked increase in these mRNA moieties within the tumor mass. These findings suggest that EGF and TGF-α may participate in the regulation of normal pancreatic exocrine function, and that overexpression of the EGF receptor and its two principal ligands may contribute to the pathophysiological processes that occur in human pancreatic cancer.
AB - The epidermal growth factor (EGF) receptor is activated by both EGF and transforming growth factor-alpha (TGF-α). Using immunohistochemical and immunoblotting techniques we now report that the EGF receptor, EGF, and TGF-α are found in both pancreatic acini and ducts in the normal human pancreas, and that all three proteins are expressed at higher levels in human pancreatic cancer tissues. Using in situ hybridization techniques, we also report that the mRNA encoding the EGF receptor, EGF, and TGF-α colocalize with their respective proteins. Northern blot analysis of total RNA indicates that, by comparison with the normal pancreas, the pancreatic tumors exhibit a 3-, 15-, and 10-fold increase in the mRNA levels encoding the EGF receptor, EGF, and TGF-α, respectively. Furthermore, by in situ hybridization, there is a marked increase in these mRNA moieties within the tumor mass. These findings suggest that EGF and TGF-α may participate in the regulation of normal pancreatic exocrine function, and that overexpression of the EGF receptor and its two principal ligands may contribute to the pathophysiological processes that occur in human pancreatic cancer.
KW - Autocrine
KW - Immunofluorescence
KW - In situ hybridization
KW - Northern blots
KW - Pancreas
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UR - http://www.scopus.com/inward/citedby.url?scp=0026462778&partnerID=8YFLogxK
M3 - Article
VL - 90
SP - 1352
EP - 1360
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
SN - 0021-9738
IS - 4
ER -