Overexpression of the epidermal growth factor receptor in human pancreatic cancer is associated with concomitant increases in the levels of epidermal growth factor and transforming growth factor alpha

Murray Korc, Bysani Chandrasekar, Yoichiro Yamanaka, Helmut Friess, Markus Buchler, Hans G. Beger

Research output: Contribution to journalArticle

467 Citations (Scopus)

Abstract

The epidermal growth factor (EGF) receptor is activated by both EGF and transforming growth factor-alpha (TGF-α). Using immunohistochemical and immunoblotting techniques we now report that the EGF receptor, EGF, and TGF-α are found in both pancreatic acini and ducts in the normal human pancreas, and that all three proteins are expressed at higher levels in human pancreatic cancer tissues. Using in situ hybridization techniques, we also report that the mRNA encoding the EGF receptor, EGF, and TGF-α colocalize with their respective proteins. Northern blot analysis of total RNA indicates that, by comparison with the normal pancreas, the pancreatic tumors exhibit a 3-, 15-, and 10-fold increase in the mRNA levels encoding the EGF receptor, EGF, and TGF-α, respectively. Furthermore, by in situ hybridization, there is a marked increase in these mRNA moieties within the tumor mass. These findings suggest that EGF and TGF-α may participate in the regulation of normal pancreatic exocrine function, and that overexpression of the EGF receptor and its two principal ligands may contribute to the pathophysiological processes that occur in human pancreatic cancer.

Original languageEnglish (US)
Pages (from-to)1352-1360
Number of pages9
JournalJournal of Clinical Investigation
Volume90
Issue number4
StatePublished - Oct 1992
Externally publishedYes

Fingerprint

Transforming Growth Factor alpha
Pancreatic Neoplasms
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Messenger RNA
In Situ Hybridization
Pancreas
Pancreatic Ducts
Immunoblotting
Northern Blotting
Neoplasms
Proteins
RNA
Ligands

Keywords

  • Autocrine
  • Immunofluorescence
  • In situ hybridization
  • Northern blots
  • Pancreas

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Overexpression of the epidermal growth factor receptor in human pancreatic cancer is associated with concomitant increases in the levels of epidermal growth factor and transforming growth factor alpha. / Korc, Murray; Chandrasekar, Bysani; Yamanaka, Yoichiro; Friess, Helmut; Buchler, Markus; Beger, Hans G.

In: Journal of Clinical Investigation, Vol. 90, No. 4, 10.1992, p. 1352-1360.

Research output: Contribution to journalArticle

Korc, Murray ; Chandrasekar, Bysani ; Yamanaka, Yoichiro ; Friess, Helmut ; Buchler, Markus ; Beger, Hans G. / Overexpression of the epidermal growth factor receptor in human pancreatic cancer is associated with concomitant increases in the levels of epidermal growth factor and transforming growth factor alpha. In: Journal of Clinical Investigation. 1992 ; Vol. 90, No. 4. pp. 1352-1360.
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