Overexpression of the P2Y2 purinoceptor in intimal lesions of the rat aorta

Cheikh Seye, Alain Pierre Gadeau, Danièle Daret, Françoise Dupuch, Philippe Alzieu, Loïc Capron, Claude Desgranges

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Extracellular nucleotides, particularly ATP, are involved in the modulation of arterial vasomotricity via P2 purinoceptors present on smooth muscle and endothelial cells. These nucleotides could also be implicated in the smooth muscle cell hyperplasia observed in intimal lesions. In this study, we tried to define the potential role of the P2Y2 (P(2u)) purinoceptor by studying its expression in normal and balloon-injured rat aortas. The cloning of a rat P2Y2 cDNA from a rat smooth muscle cell cDNA library made it possible to study P2Y2 expression both by Northern blot and in situ hybridization. Northern blot experiments indicated that P2Y2 mRNA was present in rat medial aortic smooth muscle and in cultured rat aortic smooth muscle cells. In situ hybridization indicated that P2Y2 mRNA was present in endothelial cells of the intima and in some smooth muscle cells scattered throughout the media of adult rat aortas, while almost all medial smooth muscle cells of rat embryo aorta expressed this receptor. In contrast with adult aortic media, the majority of neointimal smooth muscle cells found in aortic intimal lesions either 8 or 20 days after balloon injury were positive for P2Y2 mRNA. Moreover, a subpopulation of neointimal cells localized at the luminal surface could be identified by a higher P2Y2 expression than the underlying neointimal smooth muscle cells. These data showing a strong expression of the P2Y2 purinoceptor in the neointima of injured arteries suggest that extracellular nucleotides may be involved, via this receptor, in the intimal hyperplasia and/or chronic constriction observed at the lesion site, and consequently in the restenotic process.

Original languageEnglish (US)
Pages (from-to)3602-3610
Number of pages9
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume17
Issue number12
StatePublished - 1997
Externally publishedYes

Fingerprint

Purinergic P2Y2 Receptors
Tunica Intima
Smooth Muscle Myocytes
Aorta
Nucleotides
Northern Blotting
Messenger RNA
Hyperplasia
In Situ Hybridization
Endothelial Cells
Purinergic P2 Receptors
Purinergic Receptors
Neointima
Gene Library
Constriction
Smooth Muscle
Organism Cloning
Embryonic Structures
Complementary DNA
Arteries

Keywords

  • Balloon injury
  • Nucleotides
  • Purinoceptors
  • Rats
  • Smooth muscle cells

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Seye, C., Gadeau, A. P., Daret, D., Dupuch, F., Alzieu, P., Capron, L., & Desgranges, C. (1997). Overexpression of the P2Y2 purinoceptor in intimal lesions of the rat aorta. Arteriosclerosis, Thrombosis, and Vascular Biology, 17(12), 3602-3610.

Overexpression of the P2Y2 purinoceptor in intimal lesions of the rat aorta. / Seye, Cheikh; Gadeau, Alain Pierre; Daret, Danièle; Dupuch, Françoise; Alzieu, Philippe; Capron, Loïc; Desgranges, Claude.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 17, No. 12, 1997, p. 3602-3610.

Research output: Contribution to journalArticle

Seye, C, Gadeau, AP, Daret, D, Dupuch, F, Alzieu, P, Capron, L & Desgranges, C 1997, 'Overexpression of the P2Y2 purinoceptor in intimal lesions of the rat aorta', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 17, no. 12, pp. 3602-3610.
Seye, Cheikh ; Gadeau, Alain Pierre ; Daret, Danièle ; Dupuch, Françoise ; Alzieu, Philippe ; Capron, Loïc ; Desgranges, Claude. / Overexpression of the P2Y2 purinoceptor in intimal lesions of the rat aorta. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 1997 ; Vol. 17, No. 12. pp. 3602-3610.
@article{1b0a803bdfe241e7ab9606a1d1647010,
title = "Overexpression of the P2Y2 purinoceptor in intimal lesions of the rat aorta",
abstract = "Extracellular nucleotides, particularly ATP, are involved in the modulation of arterial vasomotricity via P2 purinoceptors present on smooth muscle and endothelial cells. These nucleotides could also be implicated in the smooth muscle cell hyperplasia observed in intimal lesions. In this study, we tried to define the potential role of the P2Y2 (P(2u)) purinoceptor by studying its expression in normal and balloon-injured rat aortas. The cloning of a rat P2Y2 cDNA from a rat smooth muscle cell cDNA library made it possible to study P2Y2 expression both by Northern blot and in situ hybridization. Northern blot experiments indicated that P2Y2 mRNA was present in rat medial aortic smooth muscle and in cultured rat aortic smooth muscle cells. In situ hybridization indicated that P2Y2 mRNA was present in endothelial cells of the intima and in some smooth muscle cells scattered throughout the media of adult rat aortas, while almost all medial smooth muscle cells of rat embryo aorta expressed this receptor. In contrast with adult aortic media, the majority of neointimal smooth muscle cells found in aortic intimal lesions either 8 or 20 days after balloon injury were positive for P2Y2 mRNA. Moreover, a subpopulation of neointimal cells localized at the luminal surface could be identified by a higher P2Y2 expression than the underlying neointimal smooth muscle cells. These data showing a strong expression of the P2Y2 purinoceptor in the neointima of injured arteries suggest that extracellular nucleotides may be involved, via this receptor, in the intimal hyperplasia and/or chronic constriction observed at the lesion site, and consequently in the restenotic process.",
keywords = "Balloon injury, Nucleotides, Purinoceptors, Rats, Smooth muscle cells",
author = "Cheikh Seye and Gadeau, {Alain Pierre} and Dani{\`e}le Daret and Fran{\cc}oise Dupuch and Philippe Alzieu and Lo{\"i}c Capron and Claude Desgranges",
year = "1997",
language = "English (US)",
volume = "17",
pages = "3602--3610",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams and Wilkins",
number = "12",

}

TY - JOUR

T1 - Overexpression of the P2Y2 purinoceptor in intimal lesions of the rat aorta

AU - Seye, Cheikh

AU - Gadeau, Alain Pierre

AU - Daret, Danièle

AU - Dupuch, Françoise

AU - Alzieu, Philippe

AU - Capron, Loïc

AU - Desgranges, Claude

PY - 1997

Y1 - 1997

N2 - Extracellular nucleotides, particularly ATP, are involved in the modulation of arterial vasomotricity via P2 purinoceptors present on smooth muscle and endothelial cells. These nucleotides could also be implicated in the smooth muscle cell hyperplasia observed in intimal lesions. In this study, we tried to define the potential role of the P2Y2 (P(2u)) purinoceptor by studying its expression in normal and balloon-injured rat aortas. The cloning of a rat P2Y2 cDNA from a rat smooth muscle cell cDNA library made it possible to study P2Y2 expression both by Northern blot and in situ hybridization. Northern blot experiments indicated that P2Y2 mRNA was present in rat medial aortic smooth muscle and in cultured rat aortic smooth muscle cells. In situ hybridization indicated that P2Y2 mRNA was present in endothelial cells of the intima and in some smooth muscle cells scattered throughout the media of adult rat aortas, while almost all medial smooth muscle cells of rat embryo aorta expressed this receptor. In contrast with adult aortic media, the majority of neointimal smooth muscle cells found in aortic intimal lesions either 8 or 20 days after balloon injury were positive for P2Y2 mRNA. Moreover, a subpopulation of neointimal cells localized at the luminal surface could be identified by a higher P2Y2 expression than the underlying neointimal smooth muscle cells. These data showing a strong expression of the P2Y2 purinoceptor in the neointima of injured arteries suggest that extracellular nucleotides may be involved, via this receptor, in the intimal hyperplasia and/or chronic constriction observed at the lesion site, and consequently in the restenotic process.

AB - Extracellular nucleotides, particularly ATP, are involved in the modulation of arterial vasomotricity via P2 purinoceptors present on smooth muscle and endothelial cells. These nucleotides could also be implicated in the smooth muscle cell hyperplasia observed in intimal lesions. In this study, we tried to define the potential role of the P2Y2 (P(2u)) purinoceptor by studying its expression in normal and balloon-injured rat aortas. The cloning of a rat P2Y2 cDNA from a rat smooth muscle cell cDNA library made it possible to study P2Y2 expression both by Northern blot and in situ hybridization. Northern blot experiments indicated that P2Y2 mRNA was present in rat medial aortic smooth muscle and in cultured rat aortic smooth muscle cells. In situ hybridization indicated that P2Y2 mRNA was present in endothelial cells of the intima and in some smooth muscle cells scattered throughout the media of adult rat aortas, while almost all medial smooth muscle cells of rat embryo aorta expressed this receptor. In contrast with adult aortic media, the majority of neointimal smooth muscle cells found in aortic intimal lesions either 8 or 20 days after balloon injury were positive for P2Y2 mRNA. Moreover, a subpopulation of neointimal cells localized at the luminal surface could be identified by a higher P2Y2 expression than the underlying neointimal smooth muscle cells. These data showing a strong expression of the P2Y2 purinoceptor in the neointima of injured arteries suggest that extracellular nucleotides may be involved, via this receptor, in the intimal hyperplasia and/or chronic constriction observed at the lesion site, and consequently in the restenotic process.

KW - Balloon injury

KW - Nucleotides

KW - Purinoceptors

KW - Rats

KW - Smooth muscle cells

UR - http://www.scopus.com/inward/record.url?scp=0031283146&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031283146&partnerID=8YFLogxK

M3 - Article

VL - 17

SP - 3602

EP - 3610

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 12

ER -