Ovulation and early embryogenesis in swine

S. Xie, D. M. Broermann, Kenneth Nephew, R. D. Geisert, W. F. Pope

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Thirty gilts were used to examine if the sequence in which oocytes were released at ovulation contributed to differences in embryonic development and uterine secretions by Day 12 (Day 0 = onset of estrus). Oocytes of follicles destined to ovulate last were recovered 42 h after injecting proestrous gilts with hCG, incubated with a fluorescent stain, and returned to the donor's oviduct. These later-maturing oocytes subsequently became the lesser-developed (p <0.01) embryos on Day 4. In a second experiment, lesser- vs. more-developed Day 4 embryos from additional gilts were transferred to ligated uterine horns of non-pregnant gilts. Subsequently, the lesser-developed Day 4 embryos became the smaller (p <0.01) blastocysts within a litter on Day 12. Uterine flushings associated with lesser-developed embryos on Day 12 contained less estradiol (p <0.01), less total protein (p <0.10), and less acid phosphatase activity (p <0.05), but total content of calcium was not different compared to flushings that contained more-developed embryos. Analysis of uterine flushings with two-dimensional PAGE procedures indicated advanced uteroferrin-associated glycoprotein secretion from the horn that contained more-developed embryos. Results of these experiments suggested that oocytes of later-ovulating follicles were progenitors of smaller embryos, which probably stimulated uterine secretion later than more advanced littermates on Day 12.

Original languageEnglish (US)
Pages (from-to)236-240
Number of pages5
JournalBiology of Reproduction
Volume43
Issue number2
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Ovulation
Embryonic Development
Swine
Embryonic Structures
Oocytes
Oviducts
Estrus
Blastocyst
Horns
Acid Phosphatase
Estradiol
Glycoproteins
Coloring Agents
Calcium
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Xie, S., Broermann, D. M., Nephew, K., Geisert, R. D., & Pope, W. F. (1990). Ovulation and early embryogenesis in swine. Biology of Reproduction, 43(2), 236-240. https://doi.org/10.1095/biolreprod43.2.236

Ovulation and early embryogenesis in swine. / Xie, S.; Broermann, D. M.; Nephew, Kenneth; Geisert, R. D.; Pope, W. F.

In: Biology of Reproduction, Vol. 43, No. 2, 1990, p. 236-240.

Research output: Contribution to journalArticle

Xie, S, Broermann, DM, Nephew, K, Geisert, RD & Pope, WF 1990, 'Ovulation and early embryogenesis in swine', Biology of Reproduction, vol. 43, no. 2, pp. 236-240. https://doi.org/10.1095/biolreprod43.2.236
Xie, S. ; Broermann, D. M. ; Nephew, Kenneth ; Geisert, R. D. ; Pope, W. F. / Ovulation and early embryogenesis in swine. In: Biology of Reproduction. 1990 ; Vol. 43, No. 2. pp. 236-240.
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