Oxaloacetate and adipose stromal cells-conditional medium synergistically protected potassium/serum deprivation-induced neuronal apoptosis

Qingpeng Liu, Gang Zhao, Changwei Zhou, Martin Farlow, Yansheng Du, Guangxu Xu, Huiying Gu

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Adipose stromal cells conditioned media (ASC-CM) protect neurons in a variety of neuronal death models including potassium/serum deprivation-induced neuronal apoptosis. In this study, we found that ASC-CM contained glutamate oxaloacetate transaminase and its substrate, oxaloacetate (OAA) directly protected cerebellar granule neurons (CGN) from apoptosis induced by serum and potassium deprivation. Additionally, OAA inhibited serum and potassium deprivation-induced caspase 3 activation. ASC-CM and OAA in combination had a synergistic neuroprotective effect. Clearly, different from ASC-CM-induced neuroprotection, OAA-induced neuroprotection was Akt- independent but JNK-dependent. These data establish a mechanistic basis supporting that the application of ASC-CM for neuroprotective treatments could be significantly enhanced by addition of OAA.

Original languageEnglish (US)
Pages (from-to)7-12
Number of pages6
JournalBrain Research Bulletin
Volume128
DOIs
StatePublished - Jan 1 2017

Keywords

  • Adipose stromal cells
  • Akt
  • Caspase-3
  • JNK
  • Neuronal apoptosis
  • Neuroprotection
  • Oxaloacetate

ASJC Scopus subject areas

  • Neuroscience(all)

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