Oxidation of cytosolic proteins and expression of creatine kinase BB in frontal lobe in different neurodegenerative disorders

Marina V. Aksenova, M. Y. Aksenov, R. Payne, J. Q. Trojanowski, M. L. Schmidt, J. M. Carney, D. A. Butterfield, W. R. Markesbery

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The presence of the biomarkers of oxidative damage, protein carbonyl formation and the inactivation of oxidatively sensitive brain creatine kinase (CK BB, cytosolic isoform), were studied in frontal lobe autopsy specimens obtained from patients with different age-related neurodegenerative diseases: Alzheimer's disease (AD), Pick's disease (PkD), diffuse Lewy body disease (DLBD), Parkinson's disease (PD), and age-matched control subjects. The CK activity was significantly reduced in the frontal lobe of AD, PkD and DLBD subjects, and CK BB-specific mRNA was significantly reduced in AD and DLBD. Protein carbonyl content was significantly increased in AD, PkD and DLBD. The results of this study confirm that the presence of biomarkers of oxidative damage is related to the presence of histopathological markers of neurodegeneration. Our data suggest that oxidative damage contributes to the development of the symptoms of frontal dysfunction in AD, PkD and DLBD. The development of frontal dysfunction in idiopathic PD might be secondary to oxidative damage and neuronal loss primarily located in the nigrostriatal system. The results of CK BB expression analysis demonstrate that the loss of the isoenzyme in different neurodegenerative diseases is likely the consequence of its posttranslational modification, possibly oxidative damage. Changes in CK BB expression may be an early indicator of oxidative stress in neurons.

Original languageEnglish (US)
Pages (from-to)158-165
Number of pages8
JournalDementia and Geriatric Cognitive Disorders
Volume10
Issue number2
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

BB Form Creatine Kinase
Lewy Body Disease
Pick Disease of the Brain
Frontal Lobe
Neurodegenerative Diseases
Alzheimer Disease
Proteins
Parkinson Disease
Biomarkers
Protein Carbonylation
Post Translational Protein Processing
Isoenzymes
Autopsy
Protein Isoforms
Oxidative Stress
Neurons
Messenger RNA

Keywords

  • Creatine kinase BB
  • Neurodegenerative disorders
  • Oxidative stress
  • Protein carbonyls

ASJC Scopus subject areas

  • Neuropsychology and Physiological Psychology
  • Geriatrics and Gerontology

Cite this

Oxidation of cytosolic proteins and expression of creatine kinase BB in frontal lobe in different neurodegenerative disorders. / Aksenova, Marina V.; Aksenov, M. Y.; Payne, R.; Trojanowski, J. Q.; Schmidt, M. L.; Carney, J. M.; Butterfield, D. A.; Markesbery, W. R.

In: Dementia and Geriatric Cognitive Disorders, Vol. 10, No. 2, 1999, p. 158-165.

Research output: Contribution to journalArticle

Aksenova, MV, Aksenov, MY, Payne, R, Trojanowski, JQ, Schmidt, ML, Carney, JM, Butterfield, DA & Markesbery, WR 1999, 'Oxidation of cytosolic proteins and expression of creatine kinase BB in frontal lobe in different neurodegenerative disorders', Dementia and Geriatric Cognitive Disorders, vol. 10, no. 2, pp. 158-165. https://doi.org/10.1159/000017098
Aksenova, Marina V. ; Aksenov, M. Y. ; Payne, R. ; Trojanowski, J. Q. ; Schmidt, M. L. ; Carney, J. M. ; Butterfield, D. A. ; Markesbery, W. R. / Oxidation of cytosolic proteins and expression of creatine kinase BB in frontal lobe in different neurodegenerative disorders. In: Dementia and Geriatric Cognitive Disorders. 1999 ; Vol. 10, No. 2. pp. 158-165.
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