Oxidative myocytes of heart and skeletal muscle express abundant sarcomeric mitochondrial creatine kinase

Wenning Qin, Zaza Khuchua, Jaime Boero, R. Payne, Arnold W. Strauss

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Sarcomeric mitochondrial creatine kinase catalyzes the reversible transfer of a high energy phosphate between ATP and creatine. To study cellular distribution of the kinase, we performed immunocytochemical studies using a peptide antiserum specific for the kinase protein. Our results demonstrated that the sarcomeric mitochondrial creatine kinase gene is abundantly expressed in heart and skeletal muscle, with no protein detected in other tissues examined, including brain, lung, liver, spleen, kidney, bladder, testis, stomach, intestine, and colon. RNA blot study showed that there is no detectable expression of the kinase mRNA in the thymus gland. In heart and skeletal muscle, the kinase protein is expressed in atrial and ventricular cardiomyocytes and a subpopulation of skeletal myofibres. In skeletal muscle, fast myosin heavy chain co-localization studies demonstrated that the sarcomeric mitochondrial creatine kinase is highly expressed in type 1, slow-oxidative and type 2A, fast-oxidative-glycolytic myofibres. We conclude that the kinase gene is abundantly expressed in oxidative myocytes of heart and skeletal muscle and may contribute to oxidative capacity of these cells.

Original languageEnglish (US)
Pages (from-to)357-365
Number of pages9
JournalHistochemical Journal
Volume31
Issue number6
DOIs
StatePublished - 1999
Externally publishedYes

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Mitochondrial Form Creatine Kinase
Muscle Cells
Myocardium
Skeletal Muscle
Phosphotransferases
Myosin Heavy Chains
Creatine
Muscle Proteins
Cardiac Myocytes
Protein Kinases
Thymus Gland
Genes
Intestines
Testis
Immune Sera
Stomach
Colon
Urinary Bladder
Spleen
Adenosine Triphosphate

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Oxidative myocytes of heart and skeletal muscle express abundant sarcomeric mitochondrial creatine kinase. / Qin, Wenning; Khuchua, Zaza; Boero, Jaime; Payne, R.; Strauss, Arnold W.

In: Histochemical Journal, Vol. 31, No. 6, 1999, p. 357-365.

Research output: Contribution to journalArticle

Qin, Wenning ; Khuchua, Zaza ; Boero, Jaime ; Payne, R. ; Strauss, Arnold W. / Oxidative myocytes of heart and skeletal muscle express abundant sarcomeric mitochondrial creatine kinase. In: Histochemical Journal. 1999 ; Vol. 31, No. 6. pp. 357-365.
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