Oxidative status in neuroblastoma: a source of stress?

Nathan M. Novotny, Jay L. Grosfeld, Katharyn E. Turner, Frederick J. Rescorla, Xinzhu Pu, James E. Klaunig, Robert J. Hickey, Linda H. Malkas, John A. Sandoval

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Aim of Study: Reactive oxygen species have been shown to be initiators/promotors of tumorigenesis. Because evidence supports the role of increased oxidative stress in solid tumors, we sought to establish this relationship in neuroblastoma (NB). The aim of the study was to investigate the extent of oxidative DNA damage and antioxidative status in a progressive animal model of human NB. Methods: Tumors were induced in the left kidneys of nude mice by the injection of cultured human NB cells (106). Blood was collected from tumor-bearing mice and controls at 2, 4, and 6 weeks. Peripheral blood leukocyte oxidative DNA damage was determined using single-cell gel electrophoresis (comet assay), and plasma antioxidant capacity was assessed by the Trolox equivalent antioxidant capacity method. Main Results: Levels of oxidative DNA damage in peripheral blood leukocytes of NB-bearing mice were increased by 166%, 110%, and 87% as compared with healthy controls at 2, 4, and 6 weeks, respectively. Plasma total antioxidant values for tumor-bearing mice were not significantly different from control mice. Conclusions: Our results indicate an increase of oxidative stress in an animal model of human NB, especially in the early stages of growth. Yet, we did not observe an appreciable response in plasma antioxidant activity. Because an altered redox status has been implicated in tumor maintenance and progression, these findings support the notion of a complex oxidant-antioxidant imbalance contributing to NB growth.

Original languageEnglish (US)
Pages (from-to)330-334
Number of pages5
JournalJournal of Pediatric Surgery
Volume43
Issue number2
DOIs
StatePublished - Feb 1 2008

Keywords

  • Animal neuroblastoma model
  • Antioxidants
  • Comet assay
  • DNA damage
  • Neuroblastoma
  • Oxidative status
  • Oxidative stress

ASJC Scopus subject areas

  • Surgery

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  • Cite this

    Novotny, N. M., Grosfeld, J. L., Turner, K. E., Rescorla, F. J., Pu, X., Klaunig, J. E., Hickey, R. J., Malkas, L. H., & Sandoval, J. A. (2008). Oxidative status in neuroblastoma: a source of stress? Journal of Pediatric Surgery, 43(2), 330-334. https://doi.org/10.1016/j.jpedsurg.2007.10.040