Oxygen modulates production of bFGF and TGF-β by retinal cells in vitro

Asud Khaliq, Bhartiben Patel, John Jarvis-Evans, Patrick Moriarty, David McLeod, Mike Boulton

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Vasoproliferative retinopathies result from retinal capillary non-perfusion and consequent inner retinal hypoxia. However, it is not known whether oxygen mediates vasoproliferation directly (at the nuclear level) or indirectly by regulating the production of growth factors. We have investigated the effect of oxygen on the production of basic fibroblast growth factor and transforming-growth-factor-beta by a variety of retinal cell types in culture. Confluent cultures were maintained for 48 hr under varying oxygen tensions ranging from 135 to 18 mmHg. A reduction in basic fibroblast growth factor levels was observed in the cell lysates and extracellular matrix from retinal microvascular endothelial cell, retinal microvascular pericyte and retinal pigment epithelial cell cultures when the oxygen tension of the medium was reduced from 135 to 18 mmHg. Levels of basic fibroblast growth factor in conditioned media from microvascular endothelial and retinal pigment epithelial cell cultures also decreased when the oxygen tension of the medium was reduced from 135 to 18 mmHg. Total transforming-growth-factor-beta (and specifically isoforms 1 and 2) in the conditioned media from all three cell types was similarly modulated by oxygen i.e. it decreased as the oxygen tension of the medium was reduced from 135 to 18 mmHg. In contrast, the steady state messenger RNA levels for both basic fibroblast growth factor and transforming-growth-factor-beta 1 genes in RPE cells increased significantly when the oxygen tension of the medium was reduced from 135 to 18 mmHg. These results support the putative role of oxygen in influencing the balance of growth factors during the development of preretinal new vessels.

Original languageEnglish (US)
Pages (from-to)415-423
Number of pages9
JournalExperimental Eye Research
Volume60
Issue number4
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Oxygen
Fibroblast Growth Factor 2
Transforming Growth Factor beta
Retinal Pigments
Cell Culture Techniques
Conditioned Culture Medium
Intercellular Signaling Peptides and Proteins
Epithelial Cells
In Vitro Techniques
Pericytes
Extracellular Matrix
Protein Isoforms
Endothelial Cells
Messenger RNA
Genes

Keywords

  • basic fibroblast growth factor
  • hypoxia
  • oxygen
  • retina
  • transforming-growth-factor-beta

ASJC Scopus subject areas

  • Ophthalmology
  • Cellular and Molecular Neuroscience
  • Sensory Systems

Cite this

Khaliq, A., Patel, B., Jarvis-Evans, J., Moriarty, P., McLeod, D., & Boulton, M. (1995). Oxygen modulates production of bFGF and TGF-β by retinal cells in vitro. Experimental Eye Research, 60(4), 415-423. https://doi.org/10.1016/S0014-4835(05)80098-3

Oxygen modulates production of bFGF and TGF-β by retinal cells in vitro. / Khaliq, Asud; Patel, Bhartiben; Jarvis-Evans, John; Moriarty, Patrick; McLeod, David; Boulton, Mike.

In: Experimental Eye Research, Vol. 60, No. 4, 1995, p. 415-423.

Research output: Contribution to journalArticle

Khaliq, A, Patel, B, Jarvis-Evans, J, Moriarty, P, McLeod, D & Boulton, M 1995, 'Oxygen modulates production of bFGF and TGF-β by retinal cells in vitro', Experimental Eye Research, vol. 60, no. 4, pp. 415-423. https://doi.org/10.1016/S0014-4835(05)80098-3
Khaliq, Asud ; Patel, Bhartiben ; Jarvis-Evans, John ; Moriarty, Patrick ; McLeod, David ; Boulton, Mike. / Oxygen modulates production of bFGF and TGF-β by retinal cells in vitro. In: Experimental Eye Research. 1995 ; Vol. 60, No. 4. pp. 415-423.
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