Oxygen tension clamp around single neurons in vitro: a computerized method for studies on O2 deprivation

Theodore R. Cummins, Samuel K. Agulian, Gabriel G. Haddad

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Abstract

This report presents a novel computerized oxygen tension clamp for use with single neurons. One main objective for developing such a system is to be able to study the intrinsic response of single neurons to graded hypoxia. The device described in this paper is comprised of three major subsystems: (1) an oxygen monitoring subsystem, (2) a motorized perfusion subsystem and (3) a computer subsystem for data acquisition and control. The clamp uses an oxygen scavenger (sodium hydrosulfite) to decrease O2 availability. The system has many advantageous features: (a) studies on hypoxia can be carried out without using chemical metabolic inhibitors to simulate hypoxia; (b) precise control of the O2 level around a given cell is feasible for extended periods of time; (c) rapid and accurate changes in the O2 level around a cell can be made because the O2 level is continuously monitored and adjusted by a computer; (d) other cells outside of the immediate vicinity of the cell being studied are not subjected to hypoxic exposures; and (e) the system is inexpensively built around readily available components.

Original languageEnglish (US)
Pages (from-to)183-189
Number of pages7
JournalJournal of Neuroscience Methods
Volume46
Issue number3
DOIs
StatePublished - Mar 1993

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Keywords

  • Anoxia
  • Computer control
  • Hypoxia
  • Isolated neuron
  • Oxygen electrode
  • Oxygen tension

ASJC Scopus subject areas

  • Neuroscience(all)

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